arXiv subjects

Sangwon Hyun

Publications and source records attributed to Sangwon Hyun.

15 recordsLinked to original sources

arXiv2026

Change Point Detection for Cell Populations Measured via Flow Cytometry

The ocean is filled with phytoplankton that contribute as much photosynthesis as all land plants combined, making them vital to the carbon cycle and climate system. Recent advances in flow cytometry allow oceanographers to measure the optical traits of individual cells along research cruise tracks, generating single-cell resolution microbial data. In marine microbial ecology, detecting locations of abrupt changes in the environmental response of cytometric plankton distributions is an important task. This manuscript proposes a latent space Gaussian mixture-of-experts model, facilitating change point detection in replicated and clustered phytoplankton observations. Change points are identified through shifts in prior means of low-dimensional representations, with piecewise-constant structure enforced by a group-fused LASSO penalty. The optimization problem is then solved via Alternating Direction Method of Multipliers. Applied to flow cytometry data, the proposed method identifies a scientifically important change point that aligns with a transition zone between two marine provinces.

stat.ME↗

arXiv2025

Defining 3-dimensional marine provinces with phytoplankton compositions

Marine provinces rarely include fine-resolution biological data, and are often defined spatially across only latitude and longitude. Therefore, we aimed to determine how phytoplankton distributions define marine provinces across 3-dimensions (i.e., latitude, longitude, and depth). To do this, we developed a new algorithm called \texttt{bioprovince} which can be applied to compositional biological data. The algorithm first clusters compositional samples to identify spatially coherent groups of samples, then makes flexible province predictions in the broader 3d spatial grid based on environmental similarity. We applied \texttt{bioprovince} to phytoplankton Amplicon Sequencing Variants (ASVs) from five, depth-resolved ocean transects spanning north-south in the Pacific Ocean. In the surface layer of the ocean, our method agreed well with traditional Longhurst provinces. In some cases, the method revealed that with more granular taxonomic resolution afforded by ASVs, traditional Longhurst provinces were divided into smaller zones. Also, one of the major advances of this method is its ability to incorporate a third dimension, depth. Indeed, our analysis found significant depth-wise partitions throughout the Pacific with remarkable agreement in the equatorial region with the base of the euphotic zone. Our algorithm's ability to delineate 3-dimensional bioprovinces will enable scientists to discover new ecological interpretations of marine phytoplankton ecology and biogeography. Furthermore, as compositional biological data inherently exists in three spatial dimensions in nature, bioprovince is broadly applicable beyond marine plankton, offering a more holistic perspective on biological provinces across diverse environments.

stat.AP↗

arXiv2025

Subcellular proteome niche discovery using semi-supervised functional clustering

Intracellular compartmentalization of proteins underpins their function and the metabolic processes they sustain. Various mass spectrometry-based proteomics methods (subcellular spatial proteomics) now allow high throughput subcellular protein localization. Yet, the curation, analysis and interpretation of these data remain challenging, particularly in non-model organisms where establishing reliable marker proteins is difficult, and in contexts where experimental replication and subcellular fractionation are constrained. Here, we develop FSPmix, a semi-supervised functional clustering method implemented as an open-source R package, which leverages partial annotations from a subset of marker proteins to predict protein subcellular localization from subcellular spatial proteomics data. This method explicitly assumes that protein signatures vary smoothly across subcellular fractions, enabling more robust inference under low signal-to-noise data regimes. We applied FSPmix to a subcellular proteomics dataset from a marine diatom, allowing us to assign probabilistic localizations to proteins and uncover potentially new protein functions. Altogether, this work lays the foundation for more robust statistical analysis and interpretation of subcellular proteomics datasets, particularly in understudied organisms.

q-bio.QM↗

arXiv2025

Experimental Analysis of Productive Interaction Strategy with ChatGPT: User Study on Function and Project-level Code Generation Tasks

The application of Large Language Models (LLMs) is growing in the productive completion of Software Engineering tasks. Yet, studies investigating productive prompting techniques often employed a limited problem space, focusing primarily on well-known prompting patterns and targeting function-level SE practices. We identify significant gaps in real-world workflows that involve complexities beyond class-level (e.g., multi-class dependencies) and different features that can impact Human-LLM Interaction (HLI) processes in code generation. To address these issues, we designed an experiment to comprehensively analyze HLI features related to code generation productivity. Our study presents two project-level benchmark tasks that extend beyond function-level evaluations. We conducted a user study with 36 participants from diverse backgrounds, asking them to solve the assigned tasks by interacting with the GPT assistant using specific prompting patterns. We also examined participants' experiences and behavioral features during interactions by analyzing screen recordings and GPT chat logs. Our empirical investigation, based on statistical guidance, revealed (1) that three out of 15 HLI features emerged as consistently supported factors for productivity; (2) five primary guidelines for enhancing productivity for HLI processes; and (3) a taxonomy of 29 runtime and logic errors that can occur during HLI processes, along with suggested mitigation plans.

cs.SE↗

arXiv2025

Search-based Selection of Metamorphic Relations for Optimized Robustness Testing of Large Language Models

Assessing the trustworthiness of Large Language Models (LLMs), such as robustness, has garnered significant attention. Recently, metamorphic testing that defines Metamorphic Relations (MRs) has been widely applied to evaluate the robustness of LLM executions. However, the MR-based robustness testing still requires a scalable number of MRs, thereby necessitating the optimization of selecting MRs. Most extant LLM testing studies are limited to automatically generating test cases (i.e., MRs) to enhance failure detection. Additionally, most studies only considered a limited test space of single perturbation MRs in their evaluation of LLMs. In contrast, our paper proposes a search-based approach for optimizing the MR groups to maximize failure detection and minimize the LLM execution cost. Moreover, our approach covers the combinatorial perturbations in MRs, facilitating the expansion of test space in the robustness assessment. We have developed a search process and implemented four search algorithms: Single-GA, NSGA-II, SPEA2, and MOEA/D with novel encoding to solve the MR selection problem in the LLM robustness testing. We conducted comparative experiments on the four search algorithms along with a random search, using two major LLMs with primary Text-to-Text tasks. Our statistical and empirical investigation revealed two key findings: (1) the MOEA/D algorithm performed the best in optimizing the MR space for LLM robustness testing, and (2) we identified silver bullet MRs for the LLM robustness testing, which demonstrated dominant capabilities in confusing LLMs across different Text-to-Text tasks. In LLM robustness assessment, our research sheds light on the fundamental problem for optimized testing and provides insights into search-based solutions.

cs.SE↗

arXiv2025

Mixtures of Neural Network Experts with Application to Phytoplankton Flow Cytometry Data

Flow cytometry is a valuable technique that measures the optical properties of particles at a single-cell resolution. When deployed in the ocean, flow cytometry allows oceanographers to study different types of photosynthetic microbes called phytoplankton. It is of great interest to study how phytoplankton properties change in response to environmental conditions. In our work, we develop a nonlinear mixture of experts model to estimate separate regression functions for each subpopulation utilizing random-weight neural networks. Our model allows one to flexibly estimate how cell properties and relative abundances depend on environmental covariates in each segment of a heterogeneous sample, without the computational burden of backpropagation. We show that the proposed model provides superior predictive performance in simulated examples compared to a mixture of linear experts. Also, applying our model to real data, we show that our model has (1) comparable out-of-sample prediction performance, and (2) more realistic estimates of phytoplankton behavior.

stat.ME↗

arXiv2025

Trend Filtered Mixture of Experts for Automated Gating of High-Frequency Flow Cytometry Data

Ocean microbes are critical to both ocean ecosystems and the global climate. Flow cytometry, which measures cell optical properties in fluid samples, is routinely used in oceanographic research. Despite decades of accumulated data, identifying key microbial populations (a process known as ``gating'') remains a significant analytical challenge. To address this, we focus on gating multidimensional, high-frequency flow cytometry data collected {\it continuously} on board oceanographic research vessels, capturing time- and space-wise variations in the dynamic ocean. Our paper proposes a novel mixture-of-experts model in which both the gating function and the experts are given by trend filtering. The model leverages two key assumptions: (1) Each snapshot of flow cytometry data is a mixture of multivariate Gaussians and (2) the parameters of these Gaussians vary smoothly over time. Our method uses regularization and a constraint to ensure smoothness and that cluster means match biologically distinct microbe types. We demonstrate, using flow cytometry data from the North Pacific Ocean, that our proposed model accurately matches human-annotated gating and corrects significant errors.

stat.ME↗

arXiv2025

DDPT: Diffusion-Driven Prompt Tuning for Large Language Model Code Generation

Large Language Models (LLMs) have demonstrated remarkable capabilities in code generation. However, the quality of the generated code is heavily dependent on the structure and composition of the prompts used. Crafting high-quality prompts is a challenging task that requires significant knowledge and skills of prompt engineering. To advance the automation support for the prompt engineering for LLM-based code generation, we propose a novel solution Diffusion-Driven Prompt Tuning (DDPT) that learns how to generate optimal prompt embedding from Gaussian Noise to automate the prompt engineering for code generation. We evaluate the feasibility of diffusion-based optimization and abstract the optimal prompt embedding as a directional vector toward the optimal embedding. We use the code generation loss given by the LLMs to help the diffusion model capture the distribution of optimal prompt embedding during training. The trained diffusion model can build a path from the noise distribution to the optimal distribution at the sampling phrase, the evaluation result demonstrates that DDPT helps improve the prompt optimization for code generation.

cs.SE↗

arXiv2025

Sunrise III: Overview of Observatory and Instruments

In July 2024, Sunrise completed its third successful science flight. The Sunrise III observatory had been upgraded significantly after the two previous successful flights in 2009 and 2013. Three completely new instruments focus on the small-scale physical processes and their complex interaction from the deepest observable layers in the photosphere up to chromospheric heights. Previously poorly explored spectral regions and lines are exploited to paint a three-dimensional picture of the solar atmosphere with unprecedented completeness and level of detail. The full polarimetric information is captured by all three instruments to reveal the interaction between the magnetic fields and the hydrodynamic processes. Two slit-based spectropolarimeters, the Sunrise UV Spectropolarimeter and Imager (SUSI) and the Sunrise Chromospheric Infrared spectro-Polarimeter (SCIP), focus on the near-ultraviolet and the near-infrared regions respectively, and the imaging spectropolarimeter Tunable Magnetograph (TuMag) simultaneously obtains maps of the full field-of-view of $46 \times 46$ Mm$^2$ in the photosphere and the chromosphere in the visible. The instruments are operated in an orchestrated mode, benefiting from a new Image Stabilization and Light Distribution unit (ISLiD), with the Correlating Wavefront Sensor (CWS) providing the autofocus control and an image stability with a root-mean-square value smaller than 0.005''. A new gondola was constructed to significantly improve the telescope pointing stability, required to achieve uninterrupted observations over many hours. Sunrise III was launched successfully on July 10, 2024, from the Esrange Space Center near Kiruna (Sweden). It reached the landing site between the Mackenzie River and the Great Bear Lake in Canada after a flight duration of 6.5 days. In this paper, we give an overview of the Sunrise III observatory and its instruments.

astro-ph.IM↗

arXiv2023

METAL: Metamorphic Testing Framework for Analyzing Large-Language Model Qualities

Large-Language Models (LLMs) have shifted the paradigm of natural language data processing. However, their black-boxed and probabilistic characteristics can lead to potential risks in the quality of outputs in diverse LLM applications. Recent studies have tested Quality Attributes (QAs), such as robustness or fairness, of LLMs by generating adversarial input texts. However, existing studies have limited their coverage of QAs and tasks in LLMs and are difficult to extend. Additionally, these studies have only used one evaluation metric, Attack Success Rate (ASR), to assess the effectiveness of their approaches. We propose a MEtamorphic Testing for Analyzing LLMs (METAL) framework to address these issues by applying Metamorphic Testing (MT) techniques. This approach facilitates the systematic testing of LLM qualities by defining Metamorphic Relations (MRs), which serve as modularized evaluation metrics. The METAL framework can automatically generate hundreds of MRs from templates that cover various QAs and tasks. In addition, we introduced novel metrics that integrate the ASR method into the semantic qualities of text to assess the effectiveness of MRs accurately. Through the experiments conducted with three prominent LLMs, we have confirmed that the METAL framework effectively evaluates essential QAs on primary LLM tasks and reveals the quality risks in LLMs. Moreover, the newly proposed metrics can guide the optimal MRs for testing each task and suggest the most effective method for generating MRs.

cs.SE↗

arXiv2022

Ocean Mover's Distance: Using Optimal Transport for Analyzing Oceanographic Data

Remote sensing observations from satellites and global biogeochemical models have combined to revolutionize the study of ocean biogeochemical cycling, but comparing the two data streams to each other and across time remains challenging due to the strong spatial-temporal structuring of the ocean. Here, we show that the Wasserstein distance provides a powerful metric for harnessing these structured datasets for better marine ecosystem and climate predictions. Wasserstein distance complements commonly used point-wise difference methods such as the root mean squared error, by quantifying differences in terms of spatial displacement in addition to magnitude. As a test case we consider Chlorophyll (a key indicator of phytoplankton biomass) in the North-East Pacific Ocean, obtained from model simulations, in situ measurements, and satellite observations. We focus on two main applications: 1) Comparing model predictions with satellite observations, and 2) temporal evolution of Chlorophyll both seasonally and over longer time frames. Wasserstein distance successfully isolates temporal and depth variability and quantifies shifts in biogeochemical province boundaries. It also exposes relevant temporal trends in satellite Chlorophyll consistent with climate change predictions. Our study shows that optimal transport vectors underlying Wasserstein distance provide a novel visualization tool for testing models and better understanding temporal dynamics in the ocean.

stat.AP↗

arXiv2022

Modeling Cell Populations Measured By Flow Cytometry With Covariates Using Sparse Mixture of Regressions

The ocean is filled with microscopic microalgae called phytoplankton, which together are responsible for as much photosynthesis as all plants on land combined. Our ability to predict their response to the warming ocean relies on understanding how the dynamics of phytoplankton populations is influenced by changes in environmental conditions. One powerful technique to study the dynamics of phytoplankton is flow cytometry, which measures the optical properties of thousands of individual cells per second. Today, oceanographers are able to collect flow cytometry data in real-time onboard a moving ship, providing them with fine-scale resolution of the distribution of phytoplankton across thousands of kilometers. One of the current challenges is to understand how these small and large scale variations relate to environmental conditions, such as nutrient availability, temperature, light and ocean currents. In this paper, we propose a novel sparse mixture of multivariate regressions model to estimate the time-varying phytoplankton subpopulations while simultaneously identifying the specific environmental covariates that are predictive of the observed changes to these subpopulations. We demonstrate the usefulness and interpretability of the approach using both synthetic data and real observations collected on an oceanographic cruise conducted in the north-east Pacific in the spring of 2017.

stat.AP↗

arXiv2018

Post-Selection Inference for Changepoint Detection Algorithms with Application to Copy Number Variation Data

Changepoint detection methods are used in many areas of science and engineering, e.g., in the analysis of copy number variation data, to detect abnormalities in copy numbers along the genome. Despite the broad array of available tools, methodology for quantifying our uncertainty in the strength (or presence) of given changepoints, post-detection, are lacking. Post-selection inference offers a framework to fill this gap, but the most straightforward application of these methods results in low-powered tests and leaves open several important questions about practical usability. In this work, we carefully tailor post-selection inference methods towards changepoint detection, focusing as our main scientific application on copy number variation data. As for changepoint algorithms, we study binary segmentation, and two of its most popular variants, wild and circular, and the fused lasso. We implement some of the latest developments in post-selection inference theory: we use auxiliary randomization to improve power, which requires implementations of MCMC algorithms (importance sampling and hit-and-run sampling) to carry out our tests. We also provide recommendations for improving practical useability, detailed simulations, and an example analysis on array comparative genomic hybridization (CGH) data.

stat.ME↗

arXiv2016

Exact Post-Selection Inference for Changepoint Detection and Other Generalized Lasso Problems

We study tools for inference conditioned on model selection events that are defined by the generalized lasso regularization path. The generalized lasso estimate is given by the solution of a penalized least squares regression problem, where the penalty is the l1 norm of a matrix D times the coefficient vector. The generalized lasso path collects these estimates for a range of penalty parameter (λ) values. Leveraging a sequential characterization of this path from Tibshirani & Taylor (2011), and recent advances in post-selection inference from Lee et al. (2016), Tibshirani et al. (2016), we develop exact hypothesis tests and confidence intervals for linear contrasts of the underlying mean vector, conditioned on any model selection event along the generalized lasso path (assuming Gaussian errors in the observations). By inspecting specific choices of D, we obtain post-selection tests and confidence intervals for specific cases of generalized lasso estimates, such as the fused lasso, trend filtering, and the graph fused lasso. In the fused lasso case, the underlying coordinates of the mean are assigned a linear ordering, and our framework allows us to test selectively chosen breakpoints or changepoints in these mean coordinates. This is an interesting and well-studied problem with broad applications, our framework applied to the trend filtering and graph fused lasso serves several applications as well. Aside from the development of selective inference tools, we describe several practical aspects of our methods such as valid post-processing of generalized estimates before performing inference in order to improve power, and problem-specific visualization aids that may be given to the data analyst for he/she to choose linear contrasts to be tested. Many examples, both from simulated and real data sources, are presented to examine the empirical properties of our inference methods.

stat.ME↗

arXiv2014

Flexible Modeling of Epidemics with an Empirical Bayes Framework

Seasonal influenza epidemics cause consistent, considerable, widespread loss annually in terms of economic burden, morbidity, and mortality. With access to accurate and reliable forecasts of a current or upcoming influenza epidemic's behavior, policy makers can design and implement more effective countermeasures. We developed a framework for in-season forecasts of epidemics using a semiparametric Empirical Bayes framework, and applied it to predict the weekly percentage of outpatient doctors visits for influenza-like illness, as well as the season onset, duration, peak time, and peak height, with and without additional data from Google Flu Trends, as part of the CDC's 2013--2014 "Predict the Influenza Season Challenge". Previous work on epidemic modeling has focused on developing mechanistic models of disease behavior and applying time series tools to explain historical data. However, these models may not accurately capture the range of possible behaviors that we may see in the future. Our approach instead produces possibilities for the epidemic curve of the season of interest using modified versions of data from previous seasons, allowing for reasonable variations in the timing, pace, and intensity of the seasonal epidemics, as well as noise in observations. Since the framework does not make strict domain-specific assumptions, it can easily be applied to other diseases as well. Another important advantage of this method is that it produces a complete posterior distribution for any desired forecasting target, rather than mere point predictions. We report prospective influenza-like-illness forecasts that were made for the 2013--2014 U.S. influenza season, and compare the framework's cross-validated prediction error on historical data to that of a variety of simpler baseline predictors.

q-bio.PE↗