arXiv subjects

Seokhwan Ko

Publications and source records attributed to Seokhwan Ko.

4 recordsLinked to original sources

arXiv2025

Development of an Agentic AI Model for NGS Downstream Analysis Targeting Researchers with Limited Biological Background

Next-Generation Sequencing (NGS) has become a cornerstone of genomic research, yet the complexity of downstream analysis-ranging from differential expression gene (DEG) identification to biological interpretations-remains a significant barrier for researchers lacking specialized computational and biological expertise. While recent studies have introduced AI agents for RNA-seq analysis, most focus on general workflows without offering tailored interpretations or guidance for novices. To address this gap, we developed an Agentic AI model designed to automate NGS downstream analysis, provide literature-backed interpretations, and autonomously recommend advanced analytical methods. Built on the Llama 3 70B Large Language Model (LLM) and a Retrieval-Augmented Generation (RAG) framework, the model is deployed as an interactive Streamlit web application. The system integrates standard bioinformatics tools (Biopython, GSEApy, gProfiler) to execute core analyses, including DEG identification, clustering, and pathway enrichment. Uniquely, the agent utilizes RAG to query PubMed via Entrez, synthesizing biological insights and validating hypotheses with current literature. In a case study using cancer-related dataset, the model successfully identified significant DEGs, visualized clinical correlations, and derived evidence-based insights (e.g., linking BRAF mutations to prognosis), subsequently executing advanced survival modeling upon user selection. This framework democratizes bioinformatics by enabling researchers with limited backgrounds to seamlessly transition from basic data processing to advanced hypothesis testing and validation.

q-bio.GN↗

arXiv2025

Leveraging Language Models and RAG for Efficient Knowledge Discovery in Clinical Environments

Large language models (LLMs) are increasingly recognized as valuable tools across the medical environment, supporting clinical, research, and administrative workflows. However, strict privacy and network security regulations in hospital settings require that sensitive data be processed within fully local infrastructures. Within this context, we developed and evaluated a retrieval-augmented generation (RAG) system designed to recommend research collaborators based on PubMed publications authored by members of a medical institution. The system utilizes PubMedBERT for domain-specific embedding generation and a locally deployed LLaMA3 model for generative synthesis. This study demonstrates the feasibility and utility of integrating domain-specialized encoders with lightweight LLMs to support biomedical knowledge discovery under local deployment constraints.

cs.CL↗

arXiv2023

Interpretable pap smear cell representation for cervical cancer screening

Screening is critical for prevention and early detection of cervical cancer but it is time-consuming and laborious. Supervised deep convolutional neural networks have been developed to automate pap smear screening and the results are promising. However, the interest in using only normal samples to train deep neural networks has increased owing to class imbalance problems and high-labeling costs that are both prevalent in healthcare. In this study, we introduce a method to learn explainable deep cervical cell representations for pap smear cytology images based on one class classification using variational autoencoders. Findings demonstrate that a score can be calculated for cell abnormality without training models with abnormal samples and localize abnormality to interpret our results with a novel metric based on absolute difference in cross entropy in agglomerative clustering. The best model that discriminates squamous cell carcinoma (SCC) from normals gives 0.908 +- 0.003 area under operating characteristic curve (AUC) and one that discriminates high-grade epithelial lesion (HSIL) 0.920 +- 0.002 AUC. Compared to other clustering methods, our method enhances the V-measure and yields higher homogeneity scores, which more effectively isolate different abnormality regions, aiding in the interpretation of our results. Evaluation using in-house and additional open dataset show that our model can discriminate abnormality without the need of additional training of deep models.

cs.CV↗

arXiv2020

Lesion Conditional Image Generation for Improved Segmentation of Intracranial Hemorrhage from CT Images

Data augmentation can effectively resolve a scarcity of images when training machine-learning algorithms. It can make them more robust to unseen images. We present a lesion conditional Generative Adversarial Network LcGAN to generate synthetic Computed Tomography (CT) images for data augmentation. A lesion conditional image (segmented mask) is an input to both the generator and the discriminator of the LcGAN during training. The trained model generates contextual CT images based on input masks. We quantify the quality of the images by using a fully convolutional network (FCN) score and blurriness. We also train another classification network to select better synthetic images. These synthetic CT images are then augmented to our hemorrhagic lesion segmentation network. By applying this augmentation method on 2.5%, 10% and 25% of original data, segmentation improved by 12.8%, 6% and 1.6% respectively.

eess.IV↗