arXiv subjects

Simo A. Mäkiharju

Publications and source records attributed to Simo A. Mäkiharju.

6 recordsLinked to original sources

arXiv2026

Time-Resolved Multi-Spectral X-ray Computed Tomography of Cryoprotectant Diffusion Into Biomimetic Material

Cryopreservation via vitrification requires loading cryoprotective cocktails. Insufficient loading may lead to freezing, precluding successful recovery; overloading is toxic. Yet, existing in situ measurements of cryoprotectant permeation remain largely unvalidated and do not resolve individual cryoprotectant concentrations. We introduce multi-spectral X-ray computed tomography (MSCT) to noninvasively quantify the spatiotemporal distribution of cryoprotectants diffusing into a tissue mimicking phantom. A developed photon-energy bin selection algorithm achieves sensitivity to low contrast cryoprotectants without contrast agents or fluorescence edges. The technique is validated with a dimethyl sulfoxide, glycerol, and water solution, resolving cryoprotectant volume fractions to within 5% accuracy. We observe heterogeneous diffusion of the cryoprotectants into the tissue mimicking hydrogel, a phenomenon not observable with conventional techniques. MSCT improves upon existing X ray CT methods because it is not underdetermined for multicomponent solutions and does not implicitly assume homogeneous diffusion. These advancements enable the systematic development of cryoprotectant loading protocols and provide diagnostics to assess vitrifiability before cryopreservation.

physics.med-ph↗

arXiv2023

In-Lab X-ray Particle Velocimetry for Multiphase Flows: Design Principles and Demonstration of $O$(1 kHz) XPV

We combine X-ray-specific tracer particles, a photon counting detector, and a liquid metal jet anode X-ray source to achieve $O$(1 kHz) X-ray imaging speeds in the laboratory, 15$\times$ faster than previous comparable studies with $O$(50 $μ$m) tracers. To examine the limits of this measurement technique we conduct three experiments: 2D and 3D X-ray particle velocimetry (XPV) of Poiseuille pipe flow, 3D XPV of flow around a Taylor bubble, and 3D scalar mixing with a laminar jet. These experiments demonstrate the performance improvement achievable by combining the aforementioned elements, the applicability to multiphase flows and deforming systems, and the potential to capture scalar and vector quantities simultaneously. Most importantly, these experiments are conducted with a laboratory-scale system, showing that in-lab X-ray particle velocimetry techniques are now becoming usable for a wider range of flows of interest. Furthermore, the design of XPV experiments is discussed to clarify the trade offs between achievable imaging speed, domain size and spatiotemporal resolution.

physics.flu-dyn↗

arXiv2023

Direct Comparison of Isobaric and Isochoric Vitrification of Two Aqueous Solutions with Photon Counting X-Ray Computed Tomography

Vitrification is a promising approach for ice-free cryopreservation of biological material, but progress is hindered by the limited set of experimental tools for studying processes in the interior of the vitrified matter. Isochoric cryopreservation chambers are often metallic, and their opacity prevents direct visual observation. In this study, we introduce photon counting X-ray computed tomography (CT) to compare the effects of rigid isochoric and unconfined isobaric conditions on vitrification and ice formation during cooling of two aqueous solutions: 50 wt% DMSO and a coral vitrification solution, CVS1. Previous studies have only compared vitrification in isochoric systems with isobaric systems that have an exposed air-liquid interface. We use a movable piston to replicate the surface and thermal boundary conditions of the isochoric system yet maintain isobaric conditions. When controlling for the boundary conditions we find that similar ice and vapor volume fractions form during cooling in isochoric and isobaric conditions. Interestingly, we observe distinct ice and vapor cavity morphology in the isochoric systems, possibly due to vapor outgassing or cavitation as rapid cooling causes the pressure to drop in the confined systems. These observations highlight the array of thermo-fluidic processes that occur during vitrification in confined aqueous systems and motivate the further application of imaging techniques such as photon counting X-ray CT in fundamental studies of vitrification.

physics.med-ph↗

arXiv2022

Enhanced Laboratory X-ray Particle Tracking Velocimetry With Newly Developed Tungsten-Coated O(50 $μ$m) Tracers

Tracer particles designed specifically for X-ray particle tracking and imaging velocimetry (XPTV and XPIV) are necessary to widen the range of flows that can be studied with these techniques. In this study, we demonstrate in-lab XPTV using new, custom-designed $O$(50 $μ$m) diameter tungsten-coated hollow carbon spheres and a single energy threshold photon counting detector. To explore the measurement quality enhancement enabled by the new tracer particles and photon counting detector, a well understood Poiseulle pipe flow is measured. The data show agreement with the analytical solution for the depth-averaged velocity profile. The experiment also shows that the tungsten-coated particles achieve higher contrast and are better localized than previously available silver-coated particles, making faster and more precise measurements attainable. The particles are manufactured with a readily scalable chemical vapor deposition process. We further show that laboratory XPTV is practical with currently available energy-resolving photon counting detectors (PCDs), despite their presently lower spatiotemporal resolution compared to scintillating detectors. This finding suggests that energy-thresholding identification of different classes of tracers is feasible, further motivating the exploration of the X-ray tracer particle design space. The latest generation of PCDs are incorporating multiple energy thresholds, and have higher count rate limits. In the near future one could potentially expand on the work presented and track multiple tracer species and scalar fields simultaneously.

physics.flu-dyn↗

arXiv2022

Experimentally Validated Simulations of 50 μm X-ray PIV Tracer Particles

We evaluate Beer-Lambert (BL) ray-tracing and Monte Carlo N-Particle (MCNP) photon tracking simulations for prediction and comparison of X-ray imaging system performance. These simulation tools can aid the methodical design of laboratory-scale X-ray particle image velocimetry (XPIV) experiments and tracer particles by predicting image quality. Particle image signal-to-noise ratio (SNR) is used as the metric of system performance. Simulated and experiment data of hollow, silver-coated, glass sphere tracer particles (AGSF-33) are compared. As predicted by the simulations, the AGSF-33 particles are visible with a SNR greater than unity in 100~ms exposure time images, demonstrating their potential as X-ray PIV or particle tracking velocimetry (XPTV) tracers. The BL approach predicts the image contrast, is computationally inexpensive, and enables the exploration of a vast parameter space for system design. MCNP simulations, on the other hand, predict experiment images slightly more accurately, but are more than an order of magnitude more computationally expensive than BL simulations. For most practical XPIV system design applications, the higher computational expense of MCNP is likely not justified by the modest accuracy improvement compared to BL.

physics.flu-dyn↗

arXiv2021

A flow disturbance estimation and rejection strategy for multirotors with round-trip trajectories

This paper presents a round-trip strategy of multirotors subject to unknown flow disturbances. During the outbound flight, the vehicle immediately utilizes the wind disturbance estimations in feedback control, as an attempt to reduce the tracking error. During this phase, the disturbance estimations with respect to the position are also recorded for future use. For the return flight, the disturbances previously collected are then routed through a feedforward controller. The major assumption here is that the disturbances may vary over space, but not over time during the same mission. We demonstrate the effectiveness of this feedforward strategy via experiments with two different types of wind flows; a simple jet flow and a more complex flow. To use as a baseline case, a cascaded PD controller with an additional feedback loop for disturbance estimation was employed for outbound flights. To display our contributions regarding the additional feedforward approach, an additional feedforward correction term obtained via prerecorded data was integrated for the return flight. Compared to the baseline controller, the feedforward controller was observed to produce 43% less RMSE position error at a vehicle ground velocity of 1 m/s with 6 m/s of environmental wind velocity. This feedforward approach also produced 14% less RMSE position error for the complex flows as well.

cs.RO↗