arXiv subjects

Stefano Zapperi

Publications and source records attributed to Stefano Zapperi.

At least 55 records · Page 3Linked to original sources

arXiv2015

Volume changes during active shape fluctuations in cells

Cells modify their volume in response to changes in osmotic pressure but it is usually assumed that other active shape variations do not involve significant volume fluctuations. Here we report experiments demonstrating that water transport in and out of the cell is needed for the formation of blebs, commonly observed protrusions in the plasma membrane driven by cortex contraction. We develop and simulate a model of fluid mediated membrane-cortex deformations and show that a permeable membrane is necessary for bleb formation which is otherwise impaired. Taken together our experimental and theoretical results emphasize the subtle balance between hydrodynamics and elasticity in actively driven cell morphological changes.

physics.bio-ph↗

arXiv2015

Osmotic stress affects functional properties of human melanoma cell lines

Understanding the role of microenvironment in cancer growth and metastasis is a key issue for cancer research. Here, we study the effect of osmotic pressure on the functional properties of primary and metastatic melanoma cell lines. In particular, we experimentally quantify individual cell motility and transmigration capability. We then perform a circular scratch assay to study how a cancer cell front invades an empty space. Our results show that primary melanoma cells are sensitive to a low osmotic pressure, while metastatic cells are less. To better understand the experimental results, we introduce and study a continuous model for the dynamics of a cell layer and a stochastic discrete model for cell proliferation and diffusion. The two models capture essential features of the experimental results and allow to make predictions for a wide range of experimentally measurable parameters.

physics.bio-ph↗

arXiv2015

Avalanches, loading and finite size effects in 2D amorphous plasticity: results from a finite element model

Crystalline plasticity is strongly interlinked with dislocation mechanics and nowadays is relatively well understood. Concepts and physical models of plastic deformation in amorphous materials on the other hand - where the concept of linear lattice defects is not applicable - still are lagging behind. We introduce an eigenstrain-based finite element lattice model for simulations of shear band formation and strain avalanches. Our model allows us to study the influence of surfaces and finite size effects on the statistics of avalanches. We find that even with relatively complex loading conditions and open boundary conditions, critical exponents describing avalanche statistics are unchanged, which validates the use of simpler scalar lattice-based models to study these phenomena.

cond-mat.soft↗

arXiv2014

Scalar model for frictional precursors dynamics

Recent experiments indicate that frictional sliding occurs by the nucleation of detachment fronts at the contact interface that may appear well before the onset of global sliding. This intriguing precursory activity is not accounted for by traditional friction theories but is extremely important for friction dominated geophysical phenomena such as earthquakes, landslides or avalanches. Here we simulate the onset of slip of a three dimensional elastic body resting on a surface and show that experimentally observed frictional precursors depend in a complex non-universal way on the sample geometry and the loading conditions. Our model satisfies Archard's law and Amontons' first and second laws, reproducing with remarkable precision the real contact area dynamics, the precursors' envelope dynamics prior to the transition to sliding, and the normal and shear internal stress distributions close to the slider-substrate interface. Moreover, it allows to assess which experimental features can be attributed to the elastic equilibrium, and which are attributed to the out-of-equilibrium dynamics, suggesting that precursory activity is an intrinsically quasi-static physical process. A direct calculation of the evolution of the Coulomb stress before and during precursors nucleation shows large variations across the sample, which helps to explain why earthquake forecasting methods based only on accumulated slip and Coulomb stress monitoring are often ineffective.

cond-mat.soft↗

arXiv2014

Fracture strength: Stress concentration, extreme value statistics and the fate of the Weibull distribution

The fracture strength distribution of materials is often described in terms of the Weibull law which can be derived by using extreme value statistics if elastic interactions are ignored. Here, we consider explicitly the interplay between elasticity and disorder and test the asymptotic validity of the Weibull distribution through numerical simulations of the two-dimensional random fuse model. Even when the local fracture strength follows the Weibull distribution, the global failure distribution is dictated by stress enhancement at the tip of the cracks and sometimes deviates from the Weibull law. Only in the case of a pre-existing power law distribution of crack widths do we find that the failure strength is Weibull distributed. Contrary to conventional assumptions, even in this case, the Weibull exponent can not be simply inferred from the exponent of the initial crack width distribution. Our results thus raise some concerns on the applicability of the Weibull distribution in most practical cases.

cond-mat.stat-mech↗

arXiv2014

Conformational mechanism for the stability of microtubule-kinetochore attachments

Regulating the stability of microtubule(MT)-kinetochore attachments is fundamental to avoiding mitotic errors and ensure proper chromosome segregation during cell division. While biochemical factors involved in this process have been identified, its mechanics still needs to be better understood. Here we introduce and simulate a mechanical model of MT-kinetochore interactions in which the stability of the attachment is ruled by the geometrical conformations of curling MT-protofilaments entangled in kinetochore fibrils. The model allows us to reproduce with good accuracy in vitro experimental measurements of the detachment times of yeast kinetochores from MTs under external pulling forces. Numerical simulations suggest that geometrical features of MT-protofilaments may play an important role in the switch between stable and unstable attachments.

physics.bio-ph↗

arXiv2014

Mechanical Properties of Growing Melanocytic Nevi and the Progression to Melanoma

Melanocytic nevi are benign proliferations that sometimes turn into malignant melanoma in a way that is still unclear from the biochemical and genetic point of view. Diagnostic and prognostic tools are then mostly based on dermoscopic examination and morphological analysis of histological tissues. To investigate the role of mechanics and geometry in the morpholgical dynamics of melanocytic nevi, we study a computation model for cell proliferation in a layered non-linear elastic tissue. Numerical simulations suggest that the morphology of the nevus is correlated to the initial location of the proliferating cell starting the growth process and to the mechanical properties of the tissue. Our results also support that melanocytes are subject to compressive stresses that fluctuate widely in the nevus and depend on the growth stage. Numerical simulations of cells in the epidermis releasing matrix metalloproteinases display an accelerated invasion of the dermis by destroying the basal membrane. Moreover, we suggest experimentally that osmotic stress and collagen inhibit growth in primary melanoma cells while the effect is much weaker in metastatic cells. Knowing that morphological features of nevi might also reflect geometry and mechanics rather than malignancy could be relevant for diagnostic purposes

q-bio.TO↗

arXiv2014

Avoidance and Coalescence of Delamination Patterns

Delamination of coatings and thin films from substrates generates a fascinating variety of patterns, from circular blisters to wrinkles and labyrinth domains, in a way that is not completely understood. We report on large-scale numerical simulations of the universal problem of avoidance and coalescence of delamination wrinkles, focusing on a case study of graphene sheets on patterned substrates. By nucleating and growing wrinkles in a controlled way, we are able to characterize how their interactions, mediated by long-range stress fields, determine their formation and morphology. We also study how the interplay between geometry and stresses drives a universal transition from conformation to delamination when sheets are deposited on particle-decorated substrates. Our results are directly applicable to strain engineering of graphene and also uncover universal phenomena observed at all scales, as for example in geomembrane deposition.

cond-mat.mtrl-sci↗

arXiv2013

Dislocation mutual interactions mediated by mobile impurities and the conditions for plastic instabilities

Matallic alloys, such as Al or Cu, or mild steel, display plastic instabilities in a well defined range of temperatures and deformation rates, a phenomenon known as the Portevin-Le Chatelelier (PLC) effect. The stick-slip behavior, or serration, typical of this effect is due to the discontinuous motion of dislocations as they interact with solute atoms. Here we study a simple model of interacting dislocations and show how the classical Einstein fluctuation-dissipation relation can be used to define the temperature in a range of model parameters and to construct a phase diagram of serration that can be compared to experimental results. Furthermore, performing analytical calculations and numerically integrating the equations of motion, we clarify the crucial role played by dislocation mutual interactions in serration.

cond-mat.mtrl-sci↗

arXiv2013

Avalanche localization and crossover scaling in amorphous plasticity

We perform large scale simulations of a two dimensional lattice model for amorphous plasticity with random local yield stresses and long-range quadrupolar elastic interactions. We show that as the external stress increases towards the yielding phase transition, the scaling behavior of the avalanches crosses over from mean-field theory to a different universality class. This behavior is associated with strain localization, which significantly depends on the short-range properties of the interaction kernel.

cond-mat.soft↗

arXiv2013

Growth and form of melanoma cell colonies

We study the statistical properties of melanoma cell colonies grown in vitro by analyzing the results of crystal violet assays at different concentrations of initial plated cells and for different growth times. The distribution of colony sizes is described well by a continuous time branching process. To characterize the shape fluctuations of the colonies, we compute the distribution of eccentricities. The experimental results are compared with numerical results for models of random division of elastic cells, showing that experimental results are best reproduced by restricting cell division to the outer rim of the colony. Our results serve to illustrate the wealth of information that can be extracted by a standard experimental method such as the crystal violet assay.

q-bio.QM↗

arXiv2013

Senescent Cells in Growing Tumors: Population Dynamics and Cancer Stem Cells

Tumors are defined by their intense proliferation, but sometimes cancer cells turn senescent and stop replicating. In the stochastic cancer model in which all cells are tumorigenic, senescence is seen as the result of random mutations, suggesting that it could represent a barrier to tumor growth. In the hierarchical cancer model a subset of the cells, the cancer stem cells, divide indefinitely while other cells eventually turn senescent. Here we formulate cancer growth in mathematical terms and obtain predictions for the evolution of senescence. We perform experiments in human melanoma cells which are compatible with the hierarchical model and show that senescence is a reversible process controlled by survivin. We conclude that enhancing senescence is unlikely to provide a useful therapeutic strategy to fight cancer, unless the cancer stem cells are specifically targeted

q-bio.TO↗

arXiv2013

Damage accumulation in quasi-brittle fracture

The strength of quasi-brittle materials depends on the ensemble of defects inside the sample and on the way damage accumulates before failure. Using large scale numerical simulations of the random fuse model, we investigate the evolution of the microcrack distribution that is directly related to the strength distribution and its size effects. We show that the broadening of the distribution tail originates from the dominating microcracks in each sample and is related to a tendency of crack coalescence that increases with system size. We study how the observed behavior depends on the disorder present in the sample.

cond-mat.stat-mech↗

arXiv2013

Avalanches in 2D Dislocation Systems: Plastic Yielding is not Depinning

We study the properties of strain bursts (dislocation avalanches) occurring in two-dimensional discrete dislocation dynamics models under quasistatic stress-controlled loading. Contrary to previous suggestions, the avalanche statistics differs fundamentally from predictions obtained for the depinning of elastic manifolds in quenched random media. Instead, we find an exponent τ=1 of the power-law distribution of slip or released energy, with a cut-off that increases exponentially with the applied stress and diverges with system size at all stresses. These observations demonstrate that the avalanche dynamics of 2D dislocation systems is scale-free at every applied stress and, therefore, can not be envisaged in terms of critical behavior associated with a depinning transition.

cond-mat.mtrl-sci↗

arXiv2013

Role of the sample thickness in planar crack propagation

We study the effect of the sample thickness in planar crack front propagation in a disordered elastic medium using the random fuse model. We employ different loading conditions and we test their stability with respect to crack growth. We show that the thickness induces characteristic lengths in the stress enhancement factor in front of the crack and in the stress transfer function parallel to the crack. This is reflected by a thickness-dependent crossover scale in the crack front morphology that goes from from multi-scaling to self-affine with exponents in agreement with line depinning models and experiments. Finally, we compute the distribution of crack avalanches which is shown to depend on the thickness and the loading mode.

cond-mat.dis-nn↗

arXiv2013

Universality classes and crossover scaling of Barkhausen noise in thin films

We study the dynamics of head-to-head domain walls separating in-plane domains in a disordered ferromagnetic thin film. The competition between the domain wall surface tension and dipolar interactions induces a crossover between a rough domain wall phase at short length-scales and a large-scale phase where the walls display a zigzag morphology. The two phases are characterized by different critical exponents for Barkhausen avalanche dynamics that are in quantitative agreement with experimental measurements on MnAs thin films.

cond-mat.stat-mech↗

arXiv2013

Quasi-periodic events in crystal plasticity and the self-organized avalanche oscillator

When external stresses in a system - physical, social or virtual - are relieved through impulsive events, it is natural to focus on the attributes of these avalanches. However, during the quiescent periods in between, stresses may be relieved through competing processes, such as slowly flowing water between earthquakes or thermally activated dislocation flow between plastic bursts. Such unassuming, smooth responses can have dramatic effects on the avalanche properties. Here we report a thorough experimental investigation of slowly compressed Ni microcrystals, covering three orders of magnitude in nominal strain rate, that exhibits unconventional quasi-periodic avalanche bursts and higher critical exponents as the strain rate is decreased. Our analytic and computational study naturally extends dislocation avalanche modeling to incorporate dislocation relaxations and reveals the emergence of the self-organized avalanche oscillator, a novel critical state exhibiting oscillatory approaches toward a depinning critical point. We demonstrate that the predictions of our theory are faithfully exhibited in our experiments.

cond-mat.stat-mech↗

arXiv2013

Barkhausen instabilities from labyrinthine magnetic domains

Experimental investigations of the scaling behavior of Barkhausen avalanches in out-of-plane ferromagnetic films yield widely different results for the values of the critical exponents despite similar labyrinthine domain structures, suggesting that universality may not hold for this class of materials. Analyzing a phase field model for magnetic reversal, we show that avalanche scaling is bounded by characteristic lengthscales arising from the competition between dipolar forces and exchange interactions. We compare our results with the experiments and find a good qualitative and quantitative agreement, reconciling apparent contradictions. Finally, we make some prediction, amenable to experimental verification, on the dependence of the avalanches behavior from the film thickness and disorder.

cond-mat.dis-nn↗