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V. I. Marconi

Publications and source records attributed to V. I. Marconi.

14 recordsLinked to original sources

Preventing clustering of active particles in microchannels

The trajectories of microswimmers moving in narrow channels of widths comparable to their sizes are significantly altered when they encounter another microswimmer moving in the opposite direction. The consequence of these encounters is a delay in the progress of both swimmers, which can be conceptualized as an instantaneous effective backward displacement. Similarly, the modeling of tumble events in bacteria, which occur over a finite time, can be represented as an instantaneous effective displacement in addition to a change in direction. Such effective displacements can be incorporated directly into a kinetic theory for the partial densities of swimmers moving in the channel. The linear analysis of the resulting equation yields the critical density at which clusters emerge. The methodology is then applied to the case of soil bacteria moving in long channels of cross-section 1.8~$μ$m $\times$ 1.8~$μ$m. The tracking of the swimmers permits the straightforward acquisition of the effective displacements, which in turn allows the critical density ($ρ_{\text{crit}}\simeq$ 0.10 bact/$μ$m) to be predicted prior to cluster formation. The advantage of this proposed approach is that it does not necessitate the determination of an effective density-dependent speed, which is a requisite of the standard motility-induced phase separation theory.

cond-mat.soft

Soil-mimicking microfluidic devices reveal restricted flagellar motility of Bradyrhizobium diazoefficiens under microconfinement

Bradyrhizobium diazoefficiens is a nitrogen-fixing symbiont of soybean, worldwide used as biofertilizer. This soil bacterium possesses two flagellar systems enabling it to swim in water-saturated soils. However, the motility in soil pores, which may be crucial for competitiveness for root nodulation, is difficult to predict. To address this gap, we fabricated microfluidic devices with networks of connected microchannels surrounding grains. In them, we directly visualise bacterial behaviour in transparent geometries mimicking minimalist soils-on-a-chip (SOCs). We measured the population velocities and changes of direction for two strains: the wild-type and a mutant with only a subpolar flagellum. A detailed statistical analysis revealed that both strains exhibited reduced speeds and increased changes of direction of 180$^\circ$, in channels of decreasing cross sectional area, down to a few microns in width. Interestingly, while the wild-type strain displayed faster swimming in unconfined spaces, this advantage was negated in the SOCs with the narrowest microchannels. We employed the measured motility parameters to propose a realistic model and simulate B. diazoefficiens confined dynamics being able to reproduce their behaviour, which additionally can be extended enabling further predictions for long time and macro scales. This multidisciplinary work, combining design, microfabrication, microbiology and modelling, offers useful methods to study soil bacteria and may be readily extended to other beneficial/harmful soil species.

physics.bio-ph

Damage and recovery of flagella in soil bacteria exposed to shear within long microchannels

The swimming motility of bacteria is driven by the action of bacterial flagellar motors, whose outermost structure is a long and thin helicoidal filament. When rotated, the fluid medium exerts an anisotropic viscous drag on the flagellar filaments, ultimately leading to bacterial propulsion. The flagellar filaments are protein-based flexible structures that can break due to interactions with fluid flows. Here, we study the evolution of flagellar filaments in the soil bacterium $\textit{Bradyrhizobium diazoefficiens}$ after being exposed to shear flows created in long microchannels, for shear rates between $1$ s$^{-1}$ and $10^5$ s$^{-1}$, and for durations between tens of milliseconds and minutes. We demonstrate that the average swimming speed and fraction of swimming cells decrease after exposition to shear, but both parameters can recover, at least partially, with time. These observations support the hypothesis that shear flows cut flagellar filaments but that reversibly damaged bacterial flagellar motors can be restored thanks to filament regeneration. By fitting our observations with phenomenological expressions, we obtain the individual growth rates of the two different flagellar filaments that $\textit{B. diazoefficiens}$ possesses, showing that the lateral filaments have a recovery time of about 40 min while the subpolar one requires more than 4.5 h to regrow. Our work demonstrates that simple monitoring of bacterial motility after exposition to shear can be used to characterize the process of flagellar filament breakup and growth, a phenomenon widely present in bacteria swimming in porous soil and exposed to shear flows due to rainfall and watering systems.

physics.bio-ph

Boosting micromachine studies with Stokesian Dynamics

Artificial microswimmers, nano and microrobots, are essential in many applications from engineering to biology and medicine. We present a Stokesian Dynamics study of the dynamical properties and efficiency of one of the simplest artificial swimmer, the three linked spheres swimmer (TLS), extensively shown to be an excellent and model example of a deformable micromachine. Results for two different swimming strokes are compared with an approximate solution based on point force interactions. While this approximation accurately reproduces the solutions for swimmers with long arms and strokes of small amplitude, it fails when the amplitude of the stroke is such that the spheres come close together, a condition where indeed the largest efficiencies are obtained. We find that swimmers with a "square stroke cycle" result more efficient than those with "circular stroke cycle" when the swimmer arms are long compared with the sphere radius, but the differences between the two strokes are smaller when the arms of the swimmers are short. This extended theoretical research of TLS incorporates a much precise description of the swimmer hydrodynamics, demonstrating the relevance of considering the finite size of the constitutive microswimmers spheres. This work expects to trigger future innovative steps contributing to the design of micro and nanomachines and its applications.

cond-mat.soft

Disrupting the wall accumulation of human sperm cells by artificial corrugation

Many self-propelled microorganisms are attracted to surfaces. This makes their dynamics in restricted geometries very different from that observed in the bulk. Swimming along walls is beneficial for directing and sorting cells, but may be detrimental if homogeneous populations are desired, such as in counting microchambers. In this work, we characterize the motion of human sperm cells $60 μm$ long, strongly confined to $25 μm$ shallow chambers. We investigate the nature of the cell trajectories between the confining surfaces and their accumulation near the borders. Observed cell trajectories are composed of a succession of quasi-circular and quasi-linear segments. This suggests that the cells follow a path of intermittent trappings near the top and bottom surfaces separated by stretches of quasi-free motion in between the two surfaces, as confirmed by depth resolved confocal microscopy studies. We show that the introduction of artificial petal-shaped corrugation in the lateral boundaries removes the tendency of cells to accumulate near the borders, an effect which we hypothesize may be valuable for microfluidic applications in biomedicine.

cond-mat.soft

Geometrical guidance and trapping transition of human sperm cells

The guidance of human sperm cells under confinement in quasi 2D microchambers is investigated using a purely physical method to control their distribution. Transport property measurements and simulations are performed with dilute sperm populations, for which effects of geometrical guidance and concentration are studied in detail. In particular, a trapping transition at convex angular wall features is identified and analyzed. We also show that highly efficient microratchets can be fabricated by using curved asymmetric obstacles to take advantage of the spermatozoa specific swimming strategy.

cond-mat.soft

Quantifying the sorting efficiency of self-propelled run-and-tumble swimmers by geometrical ratchets

Suitable asymmetric microstructures can be used to control the direction of motion in microorganism populations. This rectification process makes it possible to accumulate swimmers in a region of space or to sort different swimmers. Here we study numerically how the separation process depends on the specific motility strategies of the microorganisms involved. Crucial properties such as the separation efficiency and the separation time for two bacterial strains are precisely defined and evaluated. In particular, the sorting of two bacterial populations inoculated in a box consisting of a series of chambers separated by columns of asymmetric obstacles is investigated. We show how the sorting efficiency is enhanced by these obstacles and conclude that this kind of sorting can be efficiently used even when the involved populations differ only in one aspect of their swimming strategy.

physics.bio-ph

Influence of swimming strategy on microorganism separation by asymmetric obstacles

It has been shown that a nanoliter chamber separated by a wall of asymmetric obstacles can lead to an inhomogeneous distribution of self-propelled microorganisms. Although it is well established that this rectification effect arises from the interaction between the swimmers and the non-centrosymmetric pillars, here we demonstrate numerically that its efficiency is strongly dependent on the detailed dynamics of the individual microorganism. In particular, for the case of run-and-tumble dynamics, the distribution of run lengths, the rotational diffusion and the partial preservation of run orientation memory through a tumble are important factors when computing the rectification efficiency. In addition, we optimize the geometrical dimensions of the asymmetric pillars in order to maximize the swimmer concentration and we illustrate how it can be used for sorting by swimming strategy in a long array of parallel obstacles.

physics.bio-ph

Modeling Rectification Effects in Mesoscopic Superconducting Devices

We study thermal fluctuations and capacitive effects on small Josephson Junction Rings (JJR) that mimics the rectification phenomena recently observed in triangle shaped mesoscopic superconductors, due to the superposition of the field induced persistent current with the bias current. At finite temperature we predicted that the amplitude of the rectified signal depends strongly on the current contacts configuration on the JJR, in coincidence with experiments. In addition we analize the range of parameters where a closed loop of capacitive junctions is an appropriate model to explain the experimental observations. We conclude that the closed loop of weak links, a JJR, is a simple, robust and good enough model to explain the observed voltage rectification effects on mesoscopic superconducting samples for a wide range of temperature.

cond-mat.supr-con

Crossed-ratchet effects and domain wall geometrical pinning

The motion of a domain wall in a two dimensional medium is studied taking into account the internal elastic degrees of freedom of the wall and geometrical pinning produced both by holes and sample boundaries. This study is used to analyze the geometrical conditions needed for optimizing crossed ratchet effects in periodic rectangular arrays of asymmetric holes, recently observed experimentally in patterned ferromagnetic films. Geometrical calculations and numerical simulations have been used to obtain the anisotropic critical fields for depinning flat and kinked walls in rectangular arrays of triangles. The aim is to show with a generic elastic model for interfaces how to build a rectifier able to display crossed ratchet effects or effective potential landscapes for controlling the motion of interfaces or invasion fronts.

cond-mat.mtrl-sci

Voltage rectification effects in mesoscopic superconducting triangles: experiment and modelling

The interaction of externally applied currents with persistent currents induced by magnetic field in a mesoscopic triangle is investigated. As a consequence of the superposition of these currents, clear voltage rectification effects are observed. We demonstrate that the amplitude of the rectified signal strongly depends on the configurations of the current leads with the lowest signal obtained when the contacts are aligned along a median of the triangle. When the contacts are aligned off-centered compared to the geometrical center, the voltage response shows oscillations as a function of the applied field, whose sign can be controlled by shifting the contacts. These results are in full agreement with theoretical predictions for an analogous system consisting of a closed loop with a finite number of identical Josephson junctions.

cond-mat.supr-con

Crossed ratchet effects for magnetic domain wall motion

We study both experimentally and theoretically the driven motion of domain walls in extended amorphous magnetic films patterned with a periodic array of asymmetric holes. We find two crossed ratchet effects of opposite sign that change the preferred sense for domain wall propagation, depending on whether a flat or a kinked wall is moving. By solving numerically a simple $ϕ^4$-model we show that the essential physical ingredients for this effect are quite generic and could be realized in other experimental systems involving elastic interfaces moving in multidimensional ratchet potentials.

cond-mat.mtrl-sci

Diffuse interface approach to brittle fracture

We present a continuum model for the propagation of cracks and fractures in brittle materials. The components of the strain tensor $ε$ are the fundamental variables. The evolution equations are based on a free energy that reduces to that of linear elasticity for small $ε$, and accounts for cracks through energy saturation at large values of $ε$. We regularize the model by including terms dependent on gradients of $ε$ in the free energy. No additional fields others than the strain tensor $ε$ are introduced, and then the whole dynamics is perfectly defined. We validate the model in controlled cases, and present a couple of non trivial applications.

cond-mat.stat-mech

Orientational pinning and transverse voltage: Simulations and experiments in square Josephson junction arrays

We study the dependence of the transport properties of square Josephson Junctions arrays with the direction of the applied dc current, both experimentally and numerically. We present computational simulations of current-voltage curves at finite temperatures for a single vortex in the array ($Ha^2/Φ_0=f=1/L^2$), and experimental measurements in $100\times1000$ arrays under a low magnetic field corresponding to $f\approx0.02$. We find that the transverse voltage vanishes only in the directions of maximum symmetry of the square lattice: the [10] and [01] direction (parallel bias) and the [11] direction (diagonal bias). For orientations different than the symmetry directions, we find a finite transverse voltage which depends strongly on the angle $ϕ$ of the current. We find that vortex motion is pinned in the [10] direction ($ϕ=0$), meaning that the voltage response is insensitive to small changes in the orientation of the current near $ϕ=0$. We call this phenomenon orientational pinning. This leads to a finite transverse critical current for a bias at $ϕ=0$ and to a transverse voltage for a bias at $ϕ\not=0$. On the other hand, for diagonal bias in the [11] direction the behavior is highly unstable against small variations of $ϕ$, leading to a rapid change from zero transverse voltage to a large transverse voltage within a few degrees. This last behavior is in good agreement with our measurements in arrays with a quasi-diagonal current drive.

cond-mat.supr-con