arXiv subjects

Viatcheslav Berejnov

Publications and source records attributed to Viatcheslav Berejnov.

4 recordsLinked to original sources

arXiv2009

Rapid reconstruction of 3D structure of fibrous media

Characterization of transport properties of porous media is increasingly relying on computational methods that require reconstruction of the media structure. We present a simple method of constructing the 3D surface of fibrous porous media - the gas diffusion layer (GDL) used as the porous electrode in PEM fuel cells. The method is based on extending the depth-of-field on the whole attainable thickness of the GDL. A series of images of the GDL sample is recorded by the sequential movement of the sample with respect to the microscope focus. Different layers of the surface of the sample appear in focus in the different images in the series. The indexed series of the in-focus portions of the sample surface is combined into one sharp 2D image and interpolated into the 3D surface representing the surface of an original GDL sample. The method uses a conventional upright stage microscope that is operated manually, the inexpensive Helicon Focus software, and the open source MeshLab software. The accuracy of the reconstruction of the image features was found to be ~ 1% for probing depth of ~ 100 um with the corresponding image acquisition time being ~ 10 min. Applying this method we found that: i) this technique is able to reproduce 3D-projection of the GDL structure for a depth of ~ 50-100 um, ii) the depth of the deepest channel in the GDL can be as much as ~ 100-160 um, for GDL samples with a thickness ~ 200 um.

physics.ins-det↗

arXiv2009

Fractal flow patterns in hydrophobic microfluidic pore networks: experimental modeling of two-phase flow in porous electrodes

Experimental two-phase invasion percolation flow patterns were observed in hydrophobic micro-porous networks designed to model fuel cell specific porous media. In order to mimic the operational conditions encountered in the porous electrodes of polymer electrolyte membrane fuel cells (PEMFCs), micro-porous networks were fabricated with corresponding microchannel size distributions. The inlet channels were invaded homogeneously with flow rates corresponding to fuel cell current densities of 1.0 to 0.1 A/cm2 (Ca 10e-7-10e-8). A variety of fractal breakthrough patterns were observed and analyzed to quantify flooding density and geometrical diversity in terms of the total saturation, St, local saturations, s, and fractal dimension, D. It was found that St increases monotonically during the invasion process until the breakthrough point is reached, and s profiles indicate the dynamic distribution of the liquid phase during the process. Fractal analysis confirmed that the experiments fall within the flow regime of invasion percolation with trapping. In this work, we propose to correlate the fractal dimension, D, to the total saturation and use this map as a parameter for modeling liquid water transport in the GDL.

physics.flu-dyn↗

arXiv2006

Effects of cryoprotectant concentration and cooling rate on vitrification of aqueous solutions

Vitrification of aqueous cryoprotectant mixtures is essential in cryopreservation of proteins and other biological samples. We report systematic measurements of critical cryoprotective agent (CPA) concentrations required for vitrification during plunge cooling from T=295 K to T=77 K in liquid nitrogen. Measurements on fourteen common CPAs including alcohols (glycerol, methanol, isopropanol), sugars (sucrose, xylitol, dextrose, trehalose), PEGs (ethylene glycol, PEG 200, PEG 2 000, PEG 20 000), glycols (DMSO, MPD), and salt (NaCl) were performed for volumes ranging over four orders of magnitude from ~nL to 20 mkL, and covering the range of interest in protein crystallography. X-ray diffraction measurements on aqueous glycerol mixtures confirm that the polycrystalline-to-vitreous transition occurs within a span of less than 2% w/v in CPA concentration, and that the form of polycrystalline ice (hexagonal or cubic) depends on CPA concentration and cooling rate. For most of the studied cryoprotectants, the critical concentration decreases strongly with volume in the range from ~5 mkL to ~0.1 mkL, typically by a factor of two. By combining measurements of the critical concentration versus volume with cooling time versus volume, we obtain the function of greatest intrinsic physical interest: the critical CPA concentration versus cooling rate during flash cooling. These results provide a basis for more rational design of cryoprotective protocols, and should yield insight into the physics of glass formation in aqueous mixtures.

physics.bio-ph↗

arXiv2006

Enhancing protein drop stability for crystallization by chemical patterning

Motion of protein drops on crystallization media during routine handling is a major factor affecting the reproducibility of crystallization conditions. Drop stability can be enhanced by chemical patterning to more effectively pin the drop's contact line. As an example, a hydrophilic area is patterned on an initially flat hydrophobic glass slide. The drop remains confined to the hydrophilic area, and the maximum drop size that remains stable when the slide is rotated to the vertical position increases. This simple method is readily scalable and has the potential to significantly improve outcomes in hanging and sitting drop crystallization.

cond-mat.soft↗