arXiv subjects

Yansheng Liang

Publications and source records attributed to Yansheng Liang.

13 recordsLinked to original sources

arXiv2026

A unified reconstruction algorithm for reduced-frame structured illumination microscopy

Reduced-frame structured illumination microscopy (SIM) is attractive for live-cell imaging because it can improve temporal throughput and reduce photobleaching, but incomplete phase sampling makes reconstruction unstable and computationally demanding. Here we present URA-SIM, a unified reduced-acquisition framework that turns fixed reduced-frame measurements into pipeline- compatible raw stacks through model-consistent phase-domain completion. Instead of solving a large object-level inverse problem or replacing established SIM reconstruction, URA-SIM estimates the missing phase content on the low-dimensional phase-harmonic manifold required by the target modality and then delegates order separation and image formation to classical reconstruction pipeline. This design combines three practical advantages: fidelity from the SIM forward structure, lightweight online computation, and direct compatibility with existing reconstruction workflows. For 2D-SIM, URA-SIM uses the first-harmonic phase structure of three-phase SIM to estimate a shared zero-order field and complete missing phase samples by direction-wise harmonic fitting. On calibration and biological 2D-SIM data, reduced-frame reconstructions preserve resolvable structures and remain competitive on COS7 mitochondria comparison data. In live-cell COS7 mitochondria imaging, URA-SIM reconstructs each time point from five acquired raw frames and resolves mitochondrial cristae across different temporal sampling regimes. Experiments on 3D-SIM and nonlinear SIM further show that the same design principle can be transferred when the phase model and reconstruction-pipeline interface are adapted to the target modality. These results support URA-SIM as a transparent, model-consistent and computationally lightweight route from fixed reduced-frame acquisition to classical SIM reconstruction workflows.

physics.optics↗

arXiv2026

Imaginary Poynting momentum driven particle bidirectional rotation along arbitrary trajectory

Research on optical rotational manipulation leveraging the imaginary Poynting momentum (IPM) force has predominantly centered on cylindrically polarized Gaussian and annular beams. Here, we extend this framework to tightly focused cylindrically polarized structured light fields possessing closed or open arbitrary-intensity trajectories. We systematically elucidate the rotational dynamics induced by IPM in such fields, characterizing the underlying mechanical effects and trapping flexibilities. Notably, despite carrying zero net angular momentum, these fields drive microparticles into bidirectional rotation along predefined trajectories, thereby challenging conventional paradigms reliant on spin or orbital angular momentum. This IPM-mediated optical spanner offers unprecedented spatial degrees of freedom, paving the way for high-precision optical manipulation along arbitrarily tailored paths.

physics.optics↗

arXiv2026

Source localization realizes single frame super-resolution for fluorescence imaging

Existing super-resolution microscopy is often constrained by inherent trade-offs between resolution, acquisition speed, phototoxicity, and hardware complexity. Computational post-processing approaches offer a promising alternative, but they typically suffer from linearity distortion, high computational cost, reliance on pre-training data, or reconstruction artifacts. Here, we present Source Localization (SoLo), a novel single-frame super-resolution algorithm for fluorescence imaging without these limitations. Built on the principle of inferring fluorescent source positions via sampling-detection strategy, SoLo achieves non-iterative, parallelizable computation, enabling real-time live-cell imaging with high spatiotemporal resolution. The intensity linearity preservation of SoLo makes it compatible with quantitative analysis such as calcium imaging and fluorescence resonance energy transfer. We further extended this framework to 3D-SoLo for volumetric imaging and nonlinear SoLo (NL-SoLo) for high-density fluorescence fluctuation imaging. With its ease of parameter tuning and compatibility with existing imaging systems, SoLo offers an accessible solution for ordinary labs, enabling diverse biomedical imaging applications.

physics.optics↗

arXiv2024

On-demand Quick Metasurface Design with Neighborhood Attention Transformer

Metasurfaces are reshaping traditional optical paradigms and are increasingly required in complex applications that demand substantial computational resources to numerically solve Maxwell's equations-particularly for large-scale systems, inhomogeneous media, and densely packed metadevices. Conventional forward design using electromagnetic solvers is based on specific approximations, which may not effectively address complex problems. In contrast, existing inverse design methods are a stepwise process that is often time-consuming and involves repetitive computations. Here, we present an inverse design approach utilizing a surrogate Neighborhood Attention Transformer, MetaE-former, to predict the performance of metasurfaces with ultrafast speed and high accuracy. This method achieves global solutions for hundreds of nanostructures simultaneously, providing up to a 250,000-fold speedup compared with solving for individual meta-atoms based on the FDTD method. As examples, we demonstrate a binarized high-numerical-aperture (about 1.31) metalens and several optimized structured-light meta-generators. Our method significantly improves the beam shaping adaptability with metasurfaces and paves the way for fast designing of large-scale metadevices for shaping extreme light fields with high accuracy.

physics.optics↗

arXiv2024

High SNR 3D Imaging from Millimeter-scale Thick Tissues to Cellular Dynamics via Structured Illumination Microscopy

Three-dimensional (3D) fluorescence imaging provides a vital approach for study of biological tissues with intricate structures, and optical sectioning structured illumination microscopy (OS-SIM) stands out for its high imaging speed, low phototoxicity and high spatial resolution. However, OS-SIM faces the problem of low signal-to-noise ratio (SNR) when using traditional decoding algorithms, especially in thick tissues. Here we propose a Hilbert-transform decoding and space domain based high-low (HT-SHiLo) algorithm for noise suppression in OS-SIM. We demonstrate HT-SHiLo algorithm can significantly improve the SNR of optical sectioning images at rapid processing speed, and double the imaging depth in thick tissues. With our OS-SIM system, we achieve high quality 3D images of various biological samples including mouse brains, Drosophila clock neurons, organoids, and live cells. We anticipate that this approach will render OS-SIM a powerful technique for research of cellular organelles or thick tissues in 3D morphology.

physics.optics↗

arXiv2024

Imaginary Poynting momentum driven particle rotation by cylindrically polarized Gaussian beams

Imaginary Poynting momentum (IPM) provides a new degree of freedom for particle manipulation. However, the application of IPM in experiments has been largely unexplored. Here, we demonstrate the IPM driven particle rotation by cylindrically polarized Gaussian beams with no spin or orbital angular momentum. Theoretical analysis and experimental measurements demonstrate that gold microparticles will be rotated in the azimuthal direction while confined in the radial direction. We achieved controllable rotation of the particle by tuning the cylindrical polarization state. Interestingly, the transfer of IPM to a gold particle is demonstrated to be competitive with that of spin angular momentum. These findings hold promising in light-matter interactions and particle manipulations.

physics.optics↗

arXiv2024

Instant square lattice structured illumination microscopy: an optimal strategy towards photon-saving and real-time super-resolution observation

Over the past decade, structured illumination microscopy (SIM) has found its niche in super-resolution (SR) microscopy due to its fast imaging speed and low excitation intensity. However, due to the significantly higher light dose compared to wide-field microscopy and the time-consuming post-processing procedures, long-term, real-time, super-resolution observation of living cells is still out of reach for most SIM setups, which inevitably limits its routine use by cell biologists. Here, we describe square lattice SIM (SL-SIM) for long-duration live cell imaging by using the square lattice optical field as illumination, which allows continuous super-resolved observation over long periods of time. In addition, by extending the previous joint spatial-frequency reconstruction concept to SL-SIM, a high-speed reconstruction strategy is validated in the GPU environment, whose reconstruction time is even shorter than image acquisition time, thus enabling real-time observation. We have demonstrated the potential of SL-SIM on various biological applications, ranging from microtubule cytoskeleton dynamics to the interactions of mitochondrial cristae and DNAs in COS7 cells. The inherent lower light dose and user-friendly workflow of the SL-SIM could help make long-duration, real-time and super-resolved observations accessible to biological laboratories.

physics.optics↗

arXiv2023

Versatile non-diffracting beams generator based on free lens modulation

Non-diffracting beams, notable for their self-healing properties, high-localized intensity profiles over extended propagation distances, and resistance to diffraction, present significant utility across various fields. In this letter, we present a versatile and highly efficient non-diffracting beams (NDBs) generator predicated on the Fourier transformation of the focal plane field produced through the free lens modulation. We demonstrate the experimental generation of high-quality Bessel beams, polymorphic generalized NDBs, tilted NDBs, asymmetric NDBs, NDBs array and specially structured beams formed by the superposition of co-propagating beams. The versatile generalized NDBs generator is anticipated to find applications in laser processing, optical manipulation, and other fields.

physics.optics↗

arXiv2023

High-speed image reconstruction for nonlinear structured illumination microscopy

By exploiting the nonlinear responses of the fluorescent probes, the spatial resolution of structured illumination microscopy(SIM) can be further increased. However, due to the complex reconstruction process, the traditional reconstruction method of nonlinear structured illumination microscopy (NL-SIM) is relatively slow, which brings a great challenge to realizing real-time display of super-resolution results. To address these issues, an accelerated NL-SIM reconstruction algorithm was developed by extending a high-speed reconstruction framework, Joint Space and Frequency Reconstruction (JSFR) to NL-SIM. We anticipate that this algorithm will facilitate NL- SIM becoming a routine tool in biomedical laboratories.

physics.optics↗

arXiv2023

Versatile manipulation of low-refractive-index particles using customized optical building blocks

Low-refractive-index (LRI) particles play significant roles in physics, drug delivery, biomedical science, and other fields. However, they have not attained sufficient utilization in active manipulation due to the repulsive effect of light. Here, we demonstrate the establishment of optical building blocks (OBBs) to fulfill the demands of versatile manipulation of LRI particles. The OBBs are generated by assembling generalized perfect optical vortices based on the free lens modulation (FLM) method, by which the beams shape, intensity, and position can be elaborately designed with size independent of topological charge. Using the OBBs with high quality and high efficiency, we realized rotating LRI particles along arbitrary trajectories with controllable speed and parallel manipulation of multiple LRI particles. Importantly, we further achieved the sorting of LRI particles by size with specially structured OBBs. With unprecedented flexibility and quality, OBBs provide tremendous potential in optical trapping, lithography, and biomedicine.

physics.optics↗

arXiv2021

Zero-order-free complex beam shaping

The unwanted zero-order diffraction is still an issue in beam shaping using pixelated spatial light modulators. In this paper, we report a new approach for zero-order free beam shaping by designing an asymmetric triangle reflector and introducing a digital blazed grating and a digital lens to the phase hologram addressed onto the spatial light modulator. By adding the digital lens phase to the previously reported complex-amplitude coding algorithms, we realized the generation of complex beams without the burden of zero-order diffraction. We comparatively investigated the produced complex light fields using the modified complex-amplitude coding algorithms to validate the proposed method.

physics.optics↗

arXiv2020

Off-axis optical trapping and transverse spinning of metallic microparticles with a linearly polarized Gaussian beam

Optical trapping of metallic microparticles remains a big challenge because of the strong scattering and absorption of light by the particles. In the paper, we report a new mechanism for stable trapping of metallic microparticles by using a tightly focused linearly polarized Gaussian spot. We theoretically and experimentally demonstrated that metallic microparticles were confined off the optical axis by such a trap. In the meanwhile, transverse spinning motion occurred as a consequence of the asymmetric force field acting on the particle by the trap. The off-axis trapping and transverse spinning of metallic microparticles provide new manners for the manipulation of metallic microparticles. The works reported in this paper are also of significance for a better understanding of the mechanical interaction between light and metallic particles.

physics.optics↗

arXiv2019

Retrieval of non-sparse object through scattering media beyond the memory effect

Optical imaging through scattering media is a commonly confronted with the problem of reconstruction of complex objects and optical memory effect. To solve the problem, here, we propose a novel configuration based on the combination of ptychography and shower-curtain effect, which enables the retrieval of non-sparse samples through scattering media beyond the memory effect. Furthermore, by virtue of the shower-curtain effect, the proposed imaging system is insensitive to dynamic scattering media. Results from the retrieval of hair follicle section demonstrate the effectiveness and feasibility of the proposed method. The field of view is improved to 2.64mm. This present technique will be a potential approach for imaging through deep biological tissue.

eess.IV↗