arXiv subjects

Yiying He

Publications and source records attributed to Yiying He.

3 recordsLinked to original sources

arXiv2026

Kinetics of Mycoprotein Production from Alternative Carbon Substrates

High throughput screening was used to study of the biokinetics of F. venenatum A3/5 cultivation on alternative carbon substrates, including monosaccharides, disaccharides and mixtures relevant to food & beverage, dairy and agricultural waste streams. Expired functional drink from the beverage sector was also assessed as the primary carbon source for mycoprotein production. Growth data was analysed using modified single and multiphase Gompertz models for comparison of maximum specific growth rate and progression milestones across diverse growth regimes. Time-series substrate and byproduct data was analysed using comparative metrics, providing an explanatory basis for the different growth phenotypes observed. Substrate type strongly influenced the apparent carbon allocation strategies, with rapidly consumed sugars such as glucose and sucrose supporting high growth rates, low biomass yield and a high degree of fermentative byproduct formation. Fructose and xylose cultivations led to slower overall growth but higher biomass yield and lower byproduct formation. Galactose and lactose showed distinct dynamics that suggested co-existence of transport and metabolic induction limitations. In all dual-substrate systems, sequential utilisation was observed. However, metabolic inheritance and environmental shift effects were highlighted as potential kinetic limitations. These conditions exhibited stunted diauxic growth and low yield from secondary sugars, with glucose-dominated primary growth significantly reshaping secondary substrate efficiencies relative to their study in silo. The expired functional drink supported highly rapid growth and achieved the highest maximum specific growth rate and biomass titre of all conditions examined, alongside reduced fermentative overflow and enhanced ethanol reassimilation relative to a compositionally matched synthetic control.

physics.bio-ph↗

arXiv2025

Beyond the Expiry Date: Uncovering Hidden Value in Functional Drink Waste for a Circular Future

Expired functional drinks have great valorisation potential due to the high concentration of organic molecules present. However, detailed information of the resources in these expired functional drinks is limited, hindering the rational design of a recovery system. To address this gap, we present here a study that comprehensively characterises the chemical composition of functional drinks and discus their potential use as feedstocks for biomethane production. The example functional drinks were abundant in sugars, organic acids, and amino acids, and were especially rich in glucose, fructose, and alanine. Our studies revealed that functional drinks with high COD values that corresponded to high proportions of sugar and organic acid and low proportions of sorbitol and amino acids could realise profitable recovery through anaerobic digestion, with a minimum biomethane yield of 11.72 mL CH4 / mL drink. To assess utility further we also examined the dynamic composition of functional drinks up to 16 weeks (at 4 {\deg}C) after expiration to capture the shift in resources during deterioration. In doing so, we identified 4 distinct periods of carbon resource variation: 1) chemically stable period, 2) sorbitol degradation period, 3) sugar degradation period, and 4) acidification period. Based on the time-course biomethane production experiments for expired functional drinks, the optimal operating time window for biomethane production from drinks without ascorbic acid would be after sorbitol degradation period in terms of its economic performance through convenient natural deterioration. Therefore, this comprehensive study on dynamic chemical composition in expired functional drinks and their biomethane production potential could facilitate a rational design of resource recovery system for soft drink field.

eess.SY↗

arXiv2022

A sustainable waste-to-protein system to maximise waste resource utilisation for developing food- and feed-grade protein solutions

A waste-to-protein system that integrates a range of waste-to-protein upgrading technologies has the potential to converge innovations on zero-waste and protein security to ensure a sustainable protein future. We present a global overview of food-safe and feed-safe waste resource potential and technologies to sort and transform such waste streams with compositional quality characteristics into food-grade or feed-grade protein. The identified streams are rich in carbon and nutrients and absent of pathogens and hazardous contaminants, including food waste streams, lignocellulosic waste from agricultural residues and forestry, and contaminant-free waste from the food and drink industry. A wide range of chemical, physical, and biological treatments can be applied to extract nutrients and convert waste-carbon to fermentable sugars or other platform chemicals for subsequent conversion to protein. Our quantitative analyses suggest that the waste-to-protein system has the potential to maximise recovery of various low-value resources and catalyse the transformative solutions toward a sustainable protein future. However, novel protein regulation processes remain expensive and resource intensive in many countries, with protracted timelines for approval. This poses a significant barrier to market expansion, despite accelerated research and development in waste-to-protein technologies and novel protein sources. Thus, the waste-to-protein system is an important initiative to promote metabolic health across the lifespan and tackle the global hunger crisis.

physics.soc-ph↗