arXiv subjects

Zhangyong Li

Publications and source records attributed to Zhangyong Li.

3 recordsLinked to original sources

arXiv2025

Single-Cell Trajectory Reconstruction Reveals Migration Potential of Cell Populations

Cell migration, which is strictly regulated by intracellular and extracellular cues, is crucial for normal physiological processes and the progression of certain diseases. However, there is a lack of an efficient approach to analyze super-statistical and time-varying characteristics of cell migration based on single trajectories. Here, we propose an approach to reconstruct single-cell trajectories, which incorporates wavelet transform, power spectrum of an OU-process, and fits of the power spectrum to analyze statistical and time-varying properties of customized target-finding and migration metrics. Our results reveal diverse relationships between motility parameters and dynamic metrics, especially the existence of an optimal parameter range. Moreover, the analysis reveals that the loss of Arpin protein enhances the migration potential of D. discoideum, and a previously reported result that the rescued amoeba is distinguishable from the wild-type amoeba. Significantly, time-varying dynamic metrics emerge periodic phenomena under the influence of irregularly changing parameters, which correlates with migration potential. Our analysis suggests that the approach provides a powerful tool for estimating time-dependent migration potential and statistical features of single-cell trajectories, enabling a better understanding of the relationship between intracellular proteins and cellular behaviors. This also provides more insights on the migration dynamics of single cells and cell populations.

physics.bio-ph↗

arXiv2024

EEG-DBNet: A Dual-Branch Network for Temporal-Spectral Decoding in Motor-Imagery Brain-Computer Interfaces

Motor imagery electroencephalogram (EEG)-based brain-computer interfaces (BCIs) offer significant advantages for individuals with restricted limb mobility. However, challenges such as low signal-to-noise ratio and limited spatial resolution impede accurate feature extraction from EEG signals, thereby affecting the classification accuracy of different actions. To address these challenges, this study proposes an end-to-end dual-branch network (EEG-DBNet) that decodes the temporal and spectral sequences of EEG signals in parallel through two distinct network branches. Each branch comprises a local convolutional block and a global convolutional block. The local convolutional block transforms the source signal from the temporal-spatial domain to the temporal-spectral domain. By varying the number of filters and convolution kernel sizes, the local convolutional blocks in different branches adjust the length of their respective dimension sequences. Different types of pooling layers are then employed to emphasize the features of various dimension sequences, setting the stage for subsequent global feature extraction. The global convolution block splits and reconstructs the feature of the signal sequence processed by the local convolution block in the same branch and further extracts features through the dilated causal convolutional neural networks. Finally, the outputs from the two branches are concatenated, and signal classification is completed via a fully connected layer. Our proposed method achieves classification accuracies of 85.84% and 91.60% on the BCI Competition 4-2a and BCI Competition 4-2b datasets, respectively, surpassing existing state-of-the-art models. The source code is available at https://github.com/xicheng105/EEG-DBNet.

cs.HC↗

arXiv2023

Morphological entropy encodes cellular migration strategies on multiple length scales

Cell migration is crucial to many physiological and pathological processes. During migration, a cell adapts its morphology, including the overall morphology and nucleus morphology, in response to various cues in complex microenvironments, e.g. topotaxis and chemotaxis. Thus, cellular morphology dynamics can encode migration strategies based on which various migration mechanisms can be inferred. However, how to decipher cell migration mechanisms encoded in the morphology dynamics remains a challenging problem. Here we introduce a novel universal metric, namely cell morphological entropy (CME), by combining parametric morphological analysis with Shannon entropy. The utility of CME, which accurately quantifies the complex cellular morphology on multiple length scales through the deviation from the perfect circular shape, is demonstrated using a variety of normal and tumorous cell lines in distinct in vitro microenvironments. Our results reveal that 1) the effects of geometric constraints on cell nucleus, 2) the emerging interplays of MCF-10A cells migrating on collagen gel, and 3) the critical transition of tumor spheroid from proliferation to invasion. The analysis indicates that the CME offers a physically interpretable and efficient tool to quantify morphology on multiple length scales in real-time, which provides more insights into cell migration, and further contributing to the understanding of the diverse behavioral modes as well as collective cell motility in more complex microenvironment.

physics.bio-ph↗