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BBR-Net: Boundary-Balanced Replay for Continual Medical Image Segmentation

Continual learning for medical image segmentation remains challenging under domain shift because replay-based methods often preserve appearance information without explicitly modeling anatomical structure. This study investigates whether structural consistency governs knowledge retention in continual cardiac ultrasound segmentation. We propose the Boundary-Balanced Replay Network (BBR-Net), which selects replay samples using boundary-aware priority and class balance to preserve anatomically informative regions. The method is evaluated on CAMUS and CardiacNet under forward (CAMUS to CardiacNet) and reverse (CardiacNet to CAMUS) task orders. In the forward setting, BBR-Net retains source-task performance close to an offline joint-training reference, while markedly reducing catastrophic forgetting and preserving competitive target-task adaptation. Ablation results show that boundary-aware prioritization contributes to retention and improves the balance between source-task preservation and target-task adaptation when combined with class-aware sampling. In contrast, the reverse setting reveals that structure-aware replay fails when initial representations are learned from noisy and structurally inconsistent data. To isolate this effect, we conduct a controlled structural perturbation analysis by progressively corrupting source-task boundaries while keeping the dataset, architecture, and training protocol fixed. Forgetting increases consistently as structural reliability decreases, suggesting that replay effectiveness is strongly influenced by the quality of stored structural information, rather than by memory capacity alone. These findings indicate that preserving anatomical structure under domain shift is a central factor in continual medical image segmentation, and that replay mechanisms should account for structural reliability to support robust knowledge retention.

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Bacterial foraging optimization based brain magnetic resonance image segmentation

Segmentation partitions an image into its constituent parts. It is essentially the pre-processing stage of image analysis and computer vision. In this work, T1 and T2 weighted brain magnetic resonance images are segmented using multilevel thresholding and bacterial foraging optimization (BFO) algorithm. The thresholds are obtained by maximizing the between class variance (multilevel Otsu method) of the image. The BFO algorithm is used to optimize the threshold searching process. The edges are then obtained from the thresholded image by comparing the intensity of each pixel with its eight connected neighbourhood. Post processing is performed to remove spurious responses in the segmented image. The proposed segmentation technique is evaluated using edge detector evaluation parameters such as figure of merit, Rand Index and variation of information. The proposed brain MR image segmentation technique outperforms the traditional edge detectors such as canny and sobel.

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Accuracy Improvement of Cell Image Segmentation Using Feedback Former

Semantic segmentation of microscopy cell images by deep learning is a significant technique. We considered that the Transformers, which have recently outperformed CNNs in image recognition, could also be improved and developed for cell image segmentation. Transformers tend to focus more on contextual information than on detailed information. This tendency leads to a lack of detailed information for segmentation. Therefore, to supplement or reinforce the missing detailed information, we hypothesized that feedback processing in the human visual cortex should be effective. Our proposed Feedback Former is a novel architecture for semantic segmentation, in which Transformers is used as an encoder and has a feedback processing mechanism. Feature maps with detailed information are fed back to the lower layers from near the output of the model to compensate for the lack of detailed information which is the weakness of Transformers and improve the segmentation accuracy. By experiments on three cell image datasets, we confirmed that our method surpasses methods without feedback, demonstrating its superior accuracy in cell image segmentation. Our method achieved higher segmentation accuracy while consuming less computational cost than conventional feedback approaches. Moreover, our method offered superior precision without simply increasing the model size of Transformer encoder, demonstrating higher accuracy with lower computational cost.

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Static Segmentation by Tracking: A Label-Efficient Approach for Fine-Grained Specimen Image Segmentation

We study image segmentation in the biological domain, particularly trait segmentation from specimen images (e.g., butterfly wing stripes, beetle elytra). This fine-grained task is crucial for understanding the biology of organisms, but it traditionally requires manually annotating segmentation masks for hundreds of images per species, making it highly labor-intensive. To address this challenge, we propose a label-efficient approach, Static Segmentation by Tracking (SST), based on a key insight: while specimens of the same species exhibit natural variation, the traits of interest show up consistently. This motivates us to concatenate specimen images into a ``pseudo-video'' and reframe trait segmentation as a tracking problem. Specifically, SST generates masks for unlabeled images by propagating annotated or predicted masks from the ``pseudo-preceding'' images. Built upon recent video segmentation models, such as Segment Anything Model 2, SST achieves high-quality trait segmentation with only one labeled image per species, marking a breakthrough in specimen image analysis. To further enhance segmentation quality, we introduce a cycle-consistent loss for fine-tuning, again requiring only one labeled image. Additionally, we demonstrate the broader potential of SST, including one-shot instance segmentation in natural images and trait-based image retrieval.

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Beyond Adapting SAM: Towards End-to-End Ultrasound Image Segmentation via Auto Prompting

End-to-end medical image segmentation is of great value for computer-aided diagnosis dominated by task-specific models, usually suffering from poor generalization. With recent breakthroughs brought by the segment anything model (SAM) for universal image segmentation, extensive efforts have been made to adapt SAM for medical imaging but still encounter two major issues: 1) severe performance degradation and limited generalization without proper adaptation, and 2) semi-automatic segmentation relying on accurate manual prompts for interaction. In this work, we propose SAMUS as a universal model tailored for ultrasound image segmentation and further enable it to work in an end-to-end manner denoted as AutoSAMUS. Specifically, in SAMUS, a parallel CNN branch is introduced to supplement local information through cross-branch attention, and a feature adapter and a position adapter are jointly used to adapt SAM from natural to ultrasound domains while reducing training complexity. AutoSAMUS is realized by introducing an auto prompt generator (APG) to replace the manual prompt encoder of SAMUS to automatically generate prompt embeddings. A comprehensive ultrasound dataset, comprising about 30k images and 69k masks and covering six object categories, is collected for verification. Extensive comparison experiments demonstrate the superiority of SAMUS and AutoSAMUS against the state-of-the-art task-specific and SAM-based foundation models. We believe the auto-prompted SAM-based model has the potential to become a new paradigm for end-to-end medical image segmentation and deserves more exploration. Code and data are available at https://github.com/xianlin7/SAMUS.

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A Bottom-Up Approach to Class-Agnostic Image Segmentation

Class-agnostic image segmentation is a crucial component in automating image editing workflows, especially in contexts where object selection traditionally involves interactive tools. Existing methods in the literature often adhere to top-down formulations, following the paradigm of class-based approaches, where object detection precedes per-object segmentation. In this work, we present a novel bottom-up formulation for addressing the class-agnostic segmentation problem. We supervise our network directly on the projective sphere of its feature space, employing losses inspired by metric learning literature as well as losses defined in a novel segmentation-space representation. The segmentation results are obtained through a straightforward mean-shift clustering of the estimated features. Our bottom-up formulation exhibits exceptional generalization capability, even when trained on datasets designed for class-based segmentation. We further showcase the effectiveness of our generic approach by addressing the challenging task of cell and nucleus segmentation. We believe that our bottom-up formulation will offer valuable insights into diverse segmentation challenges in the literature.

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Multi-rater Prompting for Ambiguous Medical Image Segmentation

Multi-rater annotations commonly occur when medical images are independently annotated by multiple experts (raters). In this paper, we tackle two challenges arisen in multi-rater annotations for medical image segmentation (called ambiguous medical image segmentation): (1) How to train a deep learning model when a group of raters produces a set of diverse but plausible annotations, and (2) how to fine-tune the model efficiently when computation resources are not available for re-training the entire model on a different dataset domain. We propose a multi-rater prompt-based approach to address these two challenges altogether. Specifically, we introduce a series of rater-aware prompts that can be plugged into the U-Net model for uncertainty estimation to handle multi-annotation cases. During the prompt-based fine-tuning process, only 0.3% of learnable parameters are required to be updated comparing to training the entire model. Further, in order to integrate expert consensus and disagreement, we explore different multi-rater incorporation strategies and design a mix-training strategy for comprehensive insight learning. Extensive experiments verify the effectiveness of our new approach for ambiguous medical image segmentation on two public datasets while alleviating the heavy burden of model re-training.

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Finding an Unsupervised Image Segmenter in Each of Your Deep Generative Models

Recent research has shown that numerous human-interpretable directions exist in the latent space of GANs. In this paper, we develop an automatic procedure for finding directions that lead to foreground-background image separation, and we use these directions to train an image segmentation model without human supervision. Our method is generator-agnostic, producing strong segmentation results with a wide range of different GAN architectures. Furthermore, by leveraging GANs pretrained on large datasets such as ImageNet, we are able to segment images from a range of domains without further training or finetuning. Evaluating our method on image segmentation benchmarks, we compare favorably to prior work while using neither human supervision nor access to the training data. Broadly, our results demonstrate that automatically extracting foreground-background structure from pretrained deep generative models can serve as a remarkably effective substitute for human supervision.

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Dual Convolutional LSTM Network for Referring Image Segmentation

We consider referring image segmentation. It is a problem at the intersection of computer vision and natural language understanding. Given an input image and a referring expression in the form of a natural language sentence, the goal is to segment the object of interest in the image referred by the linguistic query. To this end, we propose a dual convolutional LSTM (ConvLSTM) network to tackle this problem. Our model consists of an encoder network and a decoder network, where ConvLSTM is used in both encoder and decoder networks to capture spatial and sequential information. The encoder network extracts visual and linguistic features for each word in the expression sentence, and adopts an attention mechanism to focus on words that are more informative in the multimodal interaction. The decoder network integrates the features generated by the encoder network at multiple levels as its input and produces the final precise segmentation mask. Experimental results on four challenging datasets demonstrate that the proposed network achieves superior segmentation performance compared with other state-of-the-art methods.

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UKBOB: One Billion MRI Labeled Masks for Generalizable 3D Medical Image Segmentation

In medical imaging, the primary challenge is collecting large-scale labeled data due to privacy concerns, logistics, and high labeling costs. In this work, we present the UK Biobank Organs and Bones (UKBOB), the largest labeled dataset of body organs, comprising 51,761 MRI 3D samples (equivalent to 17.9 million 2D images) and more than 1.37 billion 2D segmentation masks of 72 organs, all based on the UK Biobank MRI dataset. We utilize automatic labeling, introduce an automated label cleaning pipeline with organ-specific filters, and manually annotate a subset of 300 MRIs with 11 abdominal classes to validate the quality (referred to as UKBOB-manual). This approach allows for scaling up the dataset collection while maintaining confidence in the labels. We further confirm the validity of the labels by demonstrating zero-shot generalization of trained models on the filtered UKBOB to other small labeled datasets from similar domains (e.g., abdominal MRI). To further mitigate the effect of noisy labels, we propose a novel method called Entropy Test-time Adaptation (ETTA) to refine the segmentation output. We use UKBOB to train a foundation model, Swin-BOB, for 3D medical image segmentation based on the Swin-UNetr architecture, achieving state-of-the-art results in several benchmarks in 3D medical imaging, including the BRATS brain MRI tumor challenge (with a 0.4% improvement) and the BTCV abdominal CT scan benchmark (with a 1.3% improvement). The pre-trained models and the code are available at https://emmanuelleb985.github.io/ukbob , and the filtered labels will be made available with the UK Biobank.

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SAM2-Adapter: Evaluating & Adapting Segment Anything 2 in Downstream Tasks: Camouflage, Shadow, Medical Image Segmentation, and More

The advent of large models, also known as foundation models, has significantly transformed the AI research landscape, with models like Segment Anything (SAM) achieving notable success in diverse image segmentation scenarios. Despite its advancements, SAM encountered limitations in handling some complex low-level segmentation tasks like camouflaged object and medical imaging. In response, in 2023, we introduced SAM-Adapter, which demonstrated improved performance on these challenging tasks. Now, with the release of Segment Anything 2 (SAM2), a successor with enhanced architecture and a larger training corpus, we reassess these challenges. This paper introduces SAM2-Adapter, the first adapter designed to overcome the persistent limitations observed in SAM2 and achieve new state-of-the-art (SOTA) results in specific downstream tasks including medical image segmentation, camouflaged (concealed) object detection, and shadow detection. SAM2-Adapter builds on the SAM-Adapter's strengths, offering enhanced generalizability and composability for diverse applications. We present extensive experimental results demonstrating SAM2-Adapter's effectiveness. We show the potential and encourage the research community to leverage the SAM2 model with our SAM2-Adapter for achieving superior segmentation outcomes. Code, pre-trained models, and data processing protocols are available at http://tianrun-chen.github.io/SAM-Adaptor/

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SD-FSMIS: Adapting Stable Diffusion for Few-Shot Medical Image Segmentation

Few-Shot Medical Image Segmentation (FSMIS) aims to segment novel object classes in medical images using only minimal annotated examples, addressing the critical challenges of data scarcity and domain shifts prevalent in medical imaging. While Diffusion Models (DM) excel in visual tasks, their potential for FSMIS remains largely unexplored. We propose that the rich visual priors learned by large-scale DMs offer a powerful foundation for a more robust and data-efficient segmentation approach. In this paper, we introduce SD-FSMIS, a novel framework designed to effectively adapt the powerful pre-trained Stable Diffusion (SD) model for the FSMIS task. Our approach repurposes its conditional generative architecture by introducing two key components: a Support-Query Interaction (SQI) and a Visual-to-Textual Condition Translator (VTCT). Specifically, SQI provides a straightforward yet powerful means of adapting SD to the FSMIS paradigm. The VTCT module translates visual cues from the support set into an implicit textual embedding that guides the diffusion model, enabling precise conditioning of the generation process. Extensive experiments demonstrate that SD-FSMIS achieves competitive results compared to state-of-the-art methods in standard settings. Surprisingly, it also demonstrated excellent generalization ability in more challenging cross-domain scenarios. These findings highlight the immense potential of adapting large-scale generative models to advance data-efficient and robust medical image segmentation.

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Adaptive Prompt Learning with SAM for Few-shot Scanning Probe Microscope Image Segmentation

The Segment Anything Model (SAM) has demonstrated strong performance in image segmentation of natural scene images. However, its effectiveness diminishes markedly when applied to specific scientific domains, such as Scanning Probe Microscope (SPM) images. This decline in accuracy can be attributed to the distinct data distribution and limited availability of the data inherent in the scientific images. On the other hand, the acquisition of adequate SPM datasets is both time-intensive and laborious as well as skill-dependent. To address these challenges, we propose an Adaptive Prompt Learning with SAM (APL-SAM) framework tailored for few-shot SPM image segmentation. Our approach incorporates two key innovations to enhance SAM: 1) An Adaptive Prompt Learning module leverages few-shot embeddings derived from limited support set to learn adaptively central representatives, serving as visual prompts. This innovation eliminates the need for time-consuming online user interactions for providing prompts, such as exhaustively marking points and bounding boxes slice by slice; 2) A multi-source, multi-level mask decoder specifically designed for few-shot SPM image segmentation is introduced, which can effectively capture the correspondence between the support and query images. To facilitate comprehensive training and evaluation, we introduce a new dataset, SPM-Seg, curated for SPM image segmentation. Extensive experiments on this dataset reveal that the proposed APL-SAM framework significantly outperforms the original SAM, achieving over a 30% improvement in terms of Dice Similarity Coefficient with only one-shot guidance. Moreover, APL-SAM surpasses state-of-the-art few-shot segmentation methods and even fully supervised approaches in performance. Code and dataset used in this study will be made available upon acceptance.

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A Novel Framework using Intuitionistic Fuzzy Logic with U-Net and U-Net++ Architecture: A case Study of MRI Bain Image Segmentation

Accurate segmentation of brain images from magnetic resonance imaging (MRI) scans plays a pivotal role in brain image analysis and the diagnosis of neurological disorders. Deep learning algorithms, particularly U-Net and U-Net++, are widely used for image segmentation. However, it finds difficult to deal with uncertainty in images. To address this challenge, this work integrates intuitionistic fuzzy logic into U-Net and U-Net++, propose a novel framework, named as IFS U-Net and IFS U-Net++. These models accept input data in an intuitionistic fuzzy representation to manage uncertainty arising from vague ness and imprecise data. This approach effectively handles tissue ambiguity caused by the partial volume effect and boundary uncertainties. To evaluate the effectiveness of IFS U-Net and IFS U-Net++, experiments are conducted on two publicly available MRI brain datasets: the Internet Brain Segmentation Repository (IBSR) and the Open Access Series of Imaging Studies (OASIS). Segmentation performance is quantitatively assessed using Accuracy, Dice Coefficient, and Intersection over Union (IoU). The results demonstrate that the proposed architectures consistently improve segmentation performance by effectively addressing uncertainty

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Deep Learning in Medical Ultrasound Image Segmentation: a Review

Applying machine learning technologies, especially deep learning, into medical image segmentation is being widely studied because of its state-of-the-art performance and results. It can be a key step to provide a reliable basis for clinical diagnosis, such as 3D reconstruction of human tissues, image-guided interventions, image analyzing and visualization. In this review article, deep-learning-based methods for ultrasound image segmentation are categorized into six main groups according to their architectures and training at first. Secondly, for each group, several current representative algorithms are selected, introduced, analyzed and summarized in detail. In addition, common evaluation methods for image segmentation and ultrasound image segmentation datasets are summarized. Further, the performance of the current methods and their evaluations are reviewed. In the end, the challenges and potential research directions for medical ultrasound image segmentation are discussed.

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Deep Learning-based Bio-Medical Image Segmentation using UNet Architecture and Transfer Learning

Image segmentation is a branch of computer vision that is widely used in real world applications including biomedical image processing. With recent advancement of deep learning, image segmentation has achieved at a very high level performance. Recently, UNet architecture is found as the core of novel deep learning segmentation methods. In this paper we implement UNet architecture from scratch with using basic blocks in Pytorch and evaluate its performance on multiple biomedical image datasets. We also use transfer learning to apply novel modified UNet segmentation packages on the biomedical image datasets. We fine tune the pre-trained transferred model with each specific dataset. We compare its performance with our fundamental UNet implementation. We show that transferred learning model has better performance in image segmentation than UNet model that is implemented from scratch.

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Robust Seed Mask Generation for Interactive Image Segmentation

In interactive medical image segmentation, anatomical structures are extracted from reconstructed volumetric images. The first iterations of user interaction traditionally consist of drawing pictorial hints as an initial estimate of the object to extract. Only after this time consuming first phase, the efficient selective refinement of current segmentation results begins. Erroneously labeled seeds, especially near the border of the object, are challenging to detect and replace for a human and may substantially impact the overall segmentation quality. We propose an automatic seeding pipeline as well as a configuration based on saliency recognition, in order to skip the time-consuming initial interaction phase during segmentation. A median Dice score of 68.22% is reached before the first user interaction on the test data set with an error rate in seeding of only 0.088%.

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MobileUtr: Revisiting the relationship between light-weight CNN and Transformer for efficient medical image segmentation

Due to the scarcity and specific imaging characteristics in medical images, light-weighting Vision Transformers (ViTs) for efficient medical image segmentation is a significant challenge, and current studies have not yet paid attention to this issue. This work revisits the relationship between CNNs and Transformers in lightweight universal networks for medical image segmentation, aiming to integrate the advantages of both worlds at the infrastructure design level. In order to leverage the inductive bias inherent in CNNs, we abstract a Transformer-like lightweight CNNs block (ConvUtr) as the patch embeddings of ViTs, feeding Transformer with denoised, non-redundant and highly condensed semantic information. Moreover, an adaptive Local-Global-Local (LGL) block is introduced to facilitate efficient local-to-global information flow exchange, maximizing Transformer's global context information extraction capabilities. Finally, we build an efficient medical image segmentation model (MobileUtr) based on CNN and Transformer. Extensive experiments on five public medical image datasets with three different modalities demonstrate the superiority of MobileUtr over the state-of-the-art methods, while boasting lighter weights and lower computational cost. Code is available at https://github.com/FengheTan9/MobileUtr.

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