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arXiv · 0907.5157

A versatile dual spot laser scanning confocal microscopy system for advanced fluorescence correlation spectroscopy analysis in living cell

P. Ferrand·M. Pianta·A. Kress·A. Aillaud·H. Rigneault·D. Marguet
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Abstract

A fluorescence correlation spectroscopy (FCS) system based on two independent measurement volumes is presented. The optical setup and data acquisition hardware are detailed, as well as a complete protocol to control the location, size and shape of the measurement volumes. A method that allows to monitor independently the excitation and collection efficiency distribution is proposed. Finally, a few examples of measurements that exploit the two spots in static and/or scanning schemes, are reported.

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P. Ferrand, M. Pianta, A. Kress, A. Aillaud, H. Rigneault, D. Marguet. 2009-07-29. A versatile dual spot laser scanning confocal microscopy system for advanced fluorescence correlation spectroscopy analysis in living cell. https://doi.org/10.1063/1.3205447

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