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arXiv · 1312.6294

Confocal supercritical angle fluorescence microscopy for cell membrane imaging

Siddharth Sivankutty·Thomas Barroca·Céline Mayet·Guillaume Dupuis·Emmanuel Fort·Sandrine Lévêque-Fort
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Abstract

We demonstrate sub-wavelength sectioning on biological samples with a conventional confocal microscope. This optical sectioning is achieved by the phenomenon of supercritical angle fuorescence, wherein only a fluorophore next to the interface of a refractive index discontinuity can emit propagating components of radiation into the so-called forbidden angles. The simplicity of this technique allows it to be integrated with a high numerical aperture confocal scanning microscope by only a simple modification on the detection channel. Confocal-SAF microscopy would be a powerful tool to achieve high resolution surface imaging, especially for membrane imaging in biological samples

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Siddharth Sivankutty, Thomas Barroca, Céline Mayet, Guillaume Dupuis, Emmanuel Fort, Sandrine Lévêque-Fort. 2013-12-21. Confocal supercritical angle fluorescence microscopy for cell membrane imaging. https://doi.org/10.1364/ol.39.000555

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