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arXiv · 1402.3211

High-Speed Coherent Raman Fingerprint Imaging of Biological Tissues

Charles H. Camp Jr.·Young Jong Lee·John M. Heddleston·Christopher M. Hartshorn·Angela R. Hight Walker·Jeremy N. Rich·Justin D. Lathia·Marcus T. Cicerone
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Abstract

We have developed a coherent Raman imaging platform using broadband coherent anti-Stokes Raman scattering (BCARS) that provides an unprecedented combination of speed, sensitivity, and spectral breadth. The system utilizes a unique configuration of laser sources that probes the Raman spectrum over 3,000 cm$^{-1}$ and generates an especially strong response in the typically weak Raman "fingerprint" region through heterodyne amplification of the anti-Stokes photons with a large nonresonant background (NRB) while maintaining high spectral resolution of $<$ 13 cm$^{-1}$. For histology and pathology, this system shows promise in highlighting major tissue components in a non-destructive, label-free manner. We demonstrate high-speed chemical imaging in two- and three-dimensional views of healthy murine liver and pancreas tissues and interfaces between xenograft brain tumors and the surrounding healthy brain matter.

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Charles H. Camp Jr., Young Jong Lee, John M. Heddleston, Christopher M. Hartshorn, Angela R. Hight Walker, Jeremy N. Rich, Justin D. Lathia, Marcus T. Cicerone. 2014-02-15. High-Speed Coherent Raman Fingerprint Imaging of Biological Tissues. https://doi.org/10.1038/nphoton.2014.145

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