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arXiv · 1502.04826

Optogenetic control of cell signaling pathway through scattering skull using wavefront shaping

Jonghee Yoon·Minji Lee·KyeoReh Lee·Nury Kim·Jin Man Kim·Jongchan Park·Chulhee Choi·Won Do Heo·YongKeun Park
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Abstract

We introduce a non-invasive approach for optogenetic regulation in biological cells through highly scattering skull tissue using wavefront shaping. The wavefront of the incident light was systematically controlled using a spatial light modulator in order to overcome multiple light-scattering in a mouse skull layer and to focus light on the target cells. We demonstrate that illumination with shaped waves enables spatiotemporal regulation of intracellular Ca2+ level at the individual-cell level.

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Jonghee Yoon, Minji Lee, KyeoReh Lee, Nury Kim, Jin Man Kim, Jongchan Park, Chulhee Choi, Won Do Heo, YongKeun Park. 2015-02-17. Optogenetic control of cell signaling pathway through scattering skull using wavefront shaping. https://doi.org/10.1038/srep13289

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