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arXiv · 1511.04836

DUDE-Seq: Fast, Flexible, and Robust Denoising for Targeted Amplicon Sequencing

Byunghan Lee·Taesup Moon·Sungroh Yoon·Tsachy Weissman
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Abstract

We consider the correction of errors from nucleotide sequences produced by next-generation targeted amplicon sequencing. The next-generation sequencing (NGS) platforms can provide a great deal of sequencing data thanks to their high throughput, but the associated error rates often tend to be high. Denoising in high-throughput sequencing has thus become a crucial process for boosting the reliability of downstream analyses. Our methodology, named DUDE-Seq, is derived from a general setting of reconstructing finite-valued source data corrupted by a discrete memoryless channel and effectively corrects substitution and homopolymer indel errors, the two major types of sequencing errors in most high-throughput targeted amplicon sequencing platforms. Our experimental studies with real and simulated datasets suggest that the proposed DUDE-Seq not only outperforms existing alternatives in terms of error-correction capability and time efficiency, but also boosts the reliability of downstream analyses. Further, the flexibility of DUDE-Seq enables its robust application to different sequencing platforms and analysis pipelines by simple updates of the noise model. DUDE-Seq is available at http://data.snu.ac.kr/pub/dude-seq.

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Byunghan Lee, Taesup Moon, Sungroh Yoon, Tsachy Weissman. 2015-11-16. DUDE-Seq: Fast, Flexible, and Robust Denoising for Targeted Amplicon Sequencing. https://arxiv.org/abs/1511.04836

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