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arXiv · 1708.08974

An aptamer-biosensor for azole class antifungal drugs

Gregory R Wiedman·Yunan Zhao·Arkady Mustaev·Jinglei Ping·Ramya Vishnubhotla·A. T. Charlie Johnson·David S Perlin
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Abstract

This report describes the development of an aptamer for sensing azole antifungal drugs for therapeutic drug monitoring. Modified Synthetic Evolution of Ligands through Exponential Enrichment (SELEX) was used to discover a DNA aptamer recognizing azole class antifungal drugs. This aptamer undergoes a secondary structural change upon binding to its target molecule as shown through fluorescence anisotropy-based binding measurements. Experiments using circular dichroism spectroscopy, revealed a unique double G-quadruplex structure that was essential and specific for binding to the azole antifungal target. Aptamer-functionalized Graphene Field Effect Transistor (GFET) devices were created and used to measure the binding of strength of azole antifungals to this surface. In total this aptamer and the supporting sensing platform could provide a valuable tool for improving the treatment of patients with invasive fungal infections.

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Gregory R Wiedman, Yunan Zhao, Arkady Mustaev, Jinglei Ping, Ramya Vishnubhotla, A. T. Charlie Johnson, David S Perlin. 2017-08-29. An aptamer-biosensor for azole class antifungal drugs. https://doi.org/10.1128/msphere.00274-17

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