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arXiv · 1710.04957

Aberration-free calibration for 3D single molecule localization microscopy

Clément Cabriel·Nicolas Bourg·Guillaume Dupuis·Sandrine Lévêque-Fort
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Abstract

We propose a straightforward sample-based technique to calibrate the axial detection in 3D single molecule localization microscopy (SMLM). Using microspheres coated with fluorescent molecules, the calibration curves of PSF-shaping- or intensity-based measurements can be obtained for any required depth range from a few hundreds of nanometers to several tens of microns. This experimental method takes into account the effect of the spherical aberration without requiring computational correction.

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Clément Cabriel, Nicolas Bourg, Guillaume Dupuis, Sandrine Lévêque-Fort. 2017-10-13. Aberration-free calibration for 3D single molecule localization microscopy. https://doi.org/10.1364/ol.43.000174

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