arXiv subjects

Clément Cabriel

Publications and source records attributed to Clément Cabriel.

4 recordsLinked to original sources

arXiv2025

Quantitative evaluation of methods to analyze motion changes in single-particle experiments

The analysis of live-cell single-molecule imaging experiments can reveal valuable information about the heterogeneity of transport processes and interactions between cell components. These characteristics are seen as motion changes in the particle trajectories. Despite the existence of multiple approaches to carry out this type of analysis, no objective assessment of these methods has been performed so far. Here, we report the results of a competition to characterize and rank the performance of these methods when analyzing the dynamic behavior of single molecules. To run this competition, we implemented a software library that simulates realistic data corresponding to widespread diffusion and interaction models, both in the form of trajectories and videos obtained in typical experimental conditions. The competition constitutes the first assessment of these methods, providing insights into the current limitations of the field, fostering the development of new approaches, and guiding researchers to identify optimal tools for analyzing their experiments.

cond-mat.soft↗

arXiv2025

Nano-resolved sensing of 3D electromagnetic fields via single emitters' extreme variation of enhanced spontaneous emission

Controlling quantum light-matter interactions at scales smaller than the diffraction limit at the single quantum emitter level is a critical challenge to the goal of advancing quantum technologies. We introduce a novel material platform that enables precise engineering of spontaneous emission changes in molecular single emitters through 3D nanofields. This platform is based on a 3D hollow plasmonic nanomaterial arranged in a square lattice, uniformly scalable to the centimeter scale while maintaining unit cell geometry. This coupled system leads to billions of Purcell-enhanced single emitters integrated into a nanodevice. Using far-field single-molecule super-resolution microscopy, we investigate emission modifications at the single-emitter level, enabling molecular position sensing with resolution surpassing the diffraction limit. By combining the nanolocalization with time correlation single photon counting, we probe molecule per molecule enhanced quantum light-matter interactions. This 3D plasmonic geometry significantly enhances light-matter interactions, revealing a broad range of lifetimes -- from nanoseconds to picoseconds -- significantly increasing the local density of states in a manner that depends on both molecular position and dipole orientation, offering extreme position sensitivity within the 3D electromagnetic landscape. By leveraging these plasmonic nanostructures and our method for measuring single-molecule Purcell-enhanced nano-resolved maps, we enable fine-tuned control of light-matter interactions. This approach enables the on-demand control of fast single-photon sources at room temperature, providing a powerful tool for molecular sensing and quantum applications at the single-emitter level.

physics.optics↗

arXiv2025

Bottom-up Iterative Anomalous Diffusion Detector (BI-ADD)

In recent years, the segmentation of short molecular trajectories with varying diffusive properties has drawn particular attention of researchers, since it allows studying the dynamics of a particle. In the past decade, machine learning methods have shown highly promising results, also in changepoint detection and segmentation tasks. Here, we introduce a novel iterative method to identify the changepoints in a molecular trajectory, i.e., frames, where the diffusive behavior of a particle changes. A trajectory in our case follows a fractional Brownian motion and we estimate the diffusive properties of the trajectories. The proposed BI-ADD combines unsupervised and supervised learning methods to detect the changepoints. Our approach can be used for the analysis of molecular trajectories at the individual level and also be extended to multiple particle tracking, which is an important challenge in fundamental biology. We validated BI-ADD in various scenarios within the framework of the AnDi2 Challenge 2024 dedicated to single particle tracking. Our method is implemented in Python and is publicly available for research purposes.

cs.LG↗

arXiv2017

Aberration-free calibration for 3D single molecule localization microscopy

We propose a straightforward sample-based technique to calibrate the axial detection in 3D single molecule localization microscopy (SMLM). Using microspheres coated with fluorescent molecules, the calibration curves of PSF-shaping- or intensity-based measurements can be obtained for any required depth range from a few hundreds of nanometers to several tens of microns. This experimental method takes into account the effect of the spherical aberration without requiring computational correction.

physics.optics↗