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arXiv · 1803.09837

Analyte-localization device for point-of-use processing of sub-millimetre areas on surfaces

Ali Oskooei·Govind Kaigala
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Abstract

We present a portable, simple-to-operate, point-of-use analyte surface localization device. We take advantage of a set of hydrodynamic design features and components that achieve passive analyte localization by means of a single vacuum input. The vacuum source can be supplied by mechanical or battery-operated vacuum sources that are portable and allow point-of-use operation in the absence of electricity. We discuss the governing hydrodynamic principle and design parameters in detail. In a case study, we demonstrate the applicability of our technology to successfully localize a solution of rhodamine on a polydimethylsiloxane (PDMS) substrate and produce sub-millimetre-sized spots via application of a mild vacuum pressure of less than 10 kPa. In addition, we demonstrate local staining of breast cancer cell blocks and on human breast cancer tissue sections.

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Ali Oskooei, Govind Kaigala. 2018-03-08. Analyte-localization device for point-of-use processing of sub-millimetre areas on surfaces. https://doi.org/10.1016/j.snb.2017.11.130

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