SAMSON: Spectral Absorption-fluorescence Microscopy System for ON-site-imaging of algae

Abstract

This paper presents SAMSON, a Spectral Absorption-fluorescence Microscopy System for ON-site-imaging of algae within a water sample. Designed to be portable and low-cost for on-site use, the optical sub-system of SAMSON consists of a mixture of low-cost optics and electronics, designed specifically to capture both fluorescent and absorption responses from a water sample. The graphical user interface (GUI) sub-system of SAMSON was designed to enable flexible visualisation of algae in the water sample in real-time, with the ability to perform fine-grained exposure control and illumination wavelength selection. We demonstrate SAMSON's capabilities by equipping the system with two fluorescent illumination sources and seven absorption illumination sources to enable the capture of multispectral data from six different algae species (three from the Cyanophyta phylum (blue-green algae) and three from the Chlorophyta phylum (green algae)). The key benefit of SAMSON is the ability to perform rapid acquisition of fluorescence and absorption data at different wavelengths and magnification levels, thus opening the door for machine learning methods to automatically identify and enumerate different algae in water samples using this rich wealth of data.