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arXiv · 1810.10786

Supervised Classification Methods for Flash X-ray single particle diffraction Imaging

Jing Liu·Gijs van der Schot·Stefan Engblom
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Abstract

Current Flash X-ray single-particle diffraction Imaging (FXI) experiments, which operate on modern X-ray Free Electron Lasers (XFELs), can record millions of interpretable diffraction patterns from individual biomolecules per day. Due to the stochastic nature of the XFELs, those patterns will to a varying degree include scatterings from contaminated samples. Also, the heterogeneity of the sample biomolecules is unavoidable and complicates data processing. Reducing the data volumes and selecting high-quality single-molecule patterns are therefore critical steps in the experimental set-up. In this paper, we present two supervised template-based learning methods for classifying FXI patterns. Our Eigen-Image and Log-Likelihood classifier can find the best-matched template for a single-molecule pattern within a few milliseconds. It is also straightforward to parallelize them so as to fully match the XFEL repetition rate, thereby enabling processing at site.

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Jing Liu, Gijs van der Schot, Stefan Engblom. 2018-10-25. Supervised Classification Methods for Flash X-ray single particle diffraction Imaging. https://doi.org/10.1364/oe.27.003884

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