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arXiv · 1903.09777

Flagella-like beating of a single microtubule

Andrej Vilfan·Smrithika Subramani·Eberhard Bodenschatz·Ramin Golestanian·Isabella Guido
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Abstract

Kinesin motors can induce a buckling instability in a microtubule with a fixed minus end. Here we show that by modifying the surface with a protein-repellent functionalization and using clusters of kinesin motors, the microtubule can exhibit persistent oscillatory motion, resembling the beating of sperm flagella. The observed period is of the order of 1 min. From the experimental images we theoretically determine a distribution of motor forces that explains the observed shapes using a maximum likelihood approach. A good agreement is achieved with a small number of motor clusters acting simultaneously on a microtubule. The tangential forces exerted by a cluster are mostly in the range 0 - 8 pN towards the microtubule minus end, indicating the action of 1 or 2 kinesin motors. The lateral forces are distributed symmetrically and mainly below 10 pN, while the lateral velocity has a strong peak around zero. Unlike well-known models for flapping filaments, kinesins are found to have a strong "pinning" effect on the beating filaments. Our results suggest new strategies to utilize molecular motors in dynamic roles that depend sensitively on the stress built-up in the system.

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Andrej Vilfan, Smrithika Subramani, Eberhard Bodenschatz, Ramin Golestanian, Isabella Guido. 2019-03-23. Flagella-like beating of a single microtubule. https://doi.org/10.1021/acs.nanolett.9b01091

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