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arXiv · 1912.04052

Dynamic full-field optical coherence tomography: 3D live-imaging of retinal organoids

Jules Scholler·Kassandra Groux·Olivier Goureau·José-Alain Sahel·Mathias Fink·Sacha Reichman·Claude Boccara·Kate Grieve
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Abstract

Optical coherence tomography offers astounding opportunities to image the complex structure of living tissue, but lacks functional information. We present dynamic full-field optical coherence tomography to image living human induced pluripotent stem cell-derived retinal organoids non-invasively. Colored images with an endogenous contrast linked to organelle motility are generated, with sub-micrometer spatial resolution and millisecond temporal resolution, opening an avenue to identify specific cell types in living tissue via their function.

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Jules Scholler, Kassandra Groux, Olivier Goureau, José-Alain Sahel, Mathias Fink, Sacha Reichman, Claude Boccara, Kate Grieve. 2019-12-05. Dynamic full-field optical coherence tomography: 3D live-imaging of retinal organoids. https://doi.org/10.1038/s41377-020-00375-8

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