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arXiv · 2001.02844

Real-time nanodiamond thermometry probing in-vivo thermogenic responses

Masazumi Fujiwara·Simo Sun·Alexander Dohms·Yushi Nishimura·Ken Suto·Yuka Takezawa·Keisuke Oshimi·Li Zhao·Nikola Sadzak·Yumi Umehara·Yoshio Teki·Naoki Komatsu·Oliver Benson·Yutaka Shikano·Eriko Kage-Nakadai
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Abstract

Real-time temperature monitoring inside living organisms provides a direct measure of their biological activities, such as homeostatic thermoregulation and energy metabolism. However, it is challenging to reduce the size of bio-compatible thermometers down to submicrometers despite their potential applications for the thermal imaging of subtissue structures with single-cell resolution. Light-emitting nanothermometers that remotely sense temperature via optical signals exhibit considerable potential in such \textit{in-vivo} high-spatial-resolution thermometry. Here, using quantum nanothermometers based on optically accessible electron spins in nanodiamonds (NDs), we demonstrate \textit{in-vivo} real-time temperature monitoring inside \textit{Caenorhabditis elegans} (\textit{C. elegans}) worms. We developed a thermometry system that can measure the temperatures of movable NDs inside live adult worms with a precision of $\pm 0.22^{\circ}{\rm C}$. Using this system, we determined the increase in temperature based on the thermogenic responses of the worms during the chemical stimuli of mitochondrial uncouplers. Our technique demonstrates sub-micrometer localization of real-time temperature information in living animals and direct identification of their pharmacological thermogenesis. The results obtained facilitate the development of a method to probe subcellular temperature variation inside living organisms and may allow for quantification of their biological activities based on their energy expenditures.

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Masazumi Fujiwara, Simo Sun, Alexander Dohms, Yushi Nishimura, Ken Suto, Yuka Takezawa, Keisuke Oshimi, Li Zhao, Nikola Sadzak, Yumi Umehara, Yoshio Teki, Naoki Komatsu, Oliver Benson, Yutaka Shikano, Eriko Kage-Nakadai. 2020-01-09. Real-time nanodiamond thermometry probing in-vivo thermogenic responses. https://doi.org/10.1126/sciadv.aba9636

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