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arXiv · 2111.03170

3D Image Super-Resolution by fluorophore fluctuations and MA-TIRF Microscopy reconstruction (3D-COL0RME)

Vasiliki Stergiopoulou·Luca Calatroni·Sébastien Schaub·Laure Blanc-Féraud
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Abstract

We propose a 3D super-resolution approach to improve both lateral and axial spatial resolution in Total Internal Reflectance Fluorescence (TIRF) imaging applications. Our approach, called 3D-COL0RME (3D - Covariance-based $\ell_0$ super-Resolution Microscopy with intensity Estimation) improves both lateral and axial resolution by combining sparsity-based modelling for precise molecule localisation and intensity estimation in the lateral plane with a 3D reconstruction procedure in the axial one using Multi-Angle TIRF (MA-TIRF). Differently from state-of-the-art approaches, 3D-COL0RME does not require the use of special equipment as it can be used with standard fluorophores. We validate 3D-COL0RME on simulated MA-TIRF blinking-type data and on challenging real MA-TIRF acquisitions, showing significant resolution improvements.

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Vasiliki Stergiopoulou, Luca Calatroni, Sébastien Schaub, Laure Blanc-Féraud. 2021-11-04. 3D Image Super-Resolution by fluorophore fluctuations and MA-TIRF Microscopy reconstruction (3D-COL0RME). https://doi.org/10.1109/isbi52829.2022.9761572.

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