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arXiv · 2201.09960

Symbiotic bacterial network structure involved in carbon and nitrogen metabolism of wood-utilizing insect larvae

Hirokuni Miyamoto·Futo Asano·Koutarou Ishizawa·Wataru Suda·Hisashi Miyamoto·Naoko Tsuji·Makiko Matsuura·Arisa Tsuboi·Chitose Ishii·Teruno Nakaguma·Chie Shindo·Tamotsu Kato·Atsushi Kurotani·Hideaki Shima·Shigeharu Moriya·Masahira Hattori·Hiroaki Kodama·Hiroshi Ohno·Jun Kikuchi
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Abstract

Effective biological utilization of wood biomass is necessary worldwide. Since several insect larvae can use wood biomass as a nutrient source, studies on their digestive mechanism are expected to speculate a novel rule in wood biomass processing. Here, the relationships of inhabitant bacteria involved in carbon and nitrogen metabolism in the intestine of beetle larvae, an insect model, are investigated. Bacterial analysis of larval feces showed enrichment of members of which could include candidates for plant growth promotion, nitrogen cycle modulation, and/or environmental protection. The abundances of these bacteria were not necessarily positively correlated with the abundance in the habitat, suggesting that they might be selectively enriched in the intestines of larvae. Further association analysis predicted that carbon and nitrogen metabolism in the intestine was affected by the presence of the other common bacteria, the populations of which were not remarkably altered in the habitat and feces. Based on hypotheses targeting these selected bacterial groups, structural estimation modeling analyses statistically suggested that their metabolism of carbon and nitrogen and their stable isotopes, {\delta}13C and {\delta}15N, may be associated with fecal enriched bacteria and other common bacteria. In addition, other causal inference analyses, such as causal mediation analysis, linear non-Gaussian acyclic model (LiNGAM), and BayesLiNGAM, did not necessarily affirm the existence of prominent bacteria involved in metabolism, implying its importance as the bacterial groups for metabolism rather than a remarkable bacterium. Thus, these observations highlight a multifaceted view of symbiotic bacterial groups utilizing carbon and nitrogen from wood biomass in insect larvae as a cultivator of potentially environmentally beneficial bacteria.

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Hirokuni Miyamoto, Futo Asano, Koutarou Ishizawa, Wataru Suda, Hisashi Miyamoto, Naoko Tsuji, Makiko Matsuura, Arisa Tsuboi, Chitose Ishii, Teruno Nakaguma, Chie Shindo, Tamotsu Kato, Atsushi Kurotani, Hideaki Shima, Shigeharu Moriya, Masahira Hattori, Hiroaki Kodama, Hiroshi Ohno, Jun Kikuchi. 2022-01-24. Symbiotic bacterial network structure involved in carbon and nitrogen metabolism of wood-utilizing insect larvae. https://doi.org/10.1016/j.scitotenv.2022.155520

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