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arXiv · 2306.00944

pgMAP: a pipeline to enable guide RNA read mapping from dual-targeting CRISPR screens

Phoebe C. R. Parrish·Daniel J. Groso·James D. Thomas·Robert K. Bradley·Alice H. Berger
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Abstract

We developed pgMAP, an analysis pipeline to map gRNA sequencing reads from dual-targeting CRISPR screens. pgMAP output includes a dual gRNA read counts table and quality control metrics including the proportion of correctly-paired reads and CRISPR library sequencing coverage across all time points and samples. pgMAP is implemented using Snakemake and is available open-source under the MIT license at https://github.com/fredhutch/pgmap_pipeline.

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Phoebe C. R. Parrish, Daniel J. Groso, James D. Thomas, Robert K. Bradley, Alice H. Berger. 2023-06-01. pgMAP: a pipeline to enable guide RNA read mapping from dual-targeting CRISPR screens. https://arxiv.org/abs/2306.00944

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