Robert K. Bradley | Searcharxiv

arXiv2023

pgMAP: a pipeline to enable guide RNA read mapping from dual-targeting CRISPR screens

We developed pgMAP, an analysis pipeline to map gRNA sequencing reads from dual-targeting CRISPR screens. pgMAP output includes a dual gRNA read counts table and quality control metrics including the proportion of correctly-paired reads and CRISPR library sequencing coverage across all time points and samples. pgMAP is implemented using Snakemake and is available open-source under the MIT license at https://github.com/fredhutch/pgmap_pipeline.

q-bio.GN↗

arXiv2004

Degenerate eigenvalues for Hamiltonians with no obvious symmetries

Certain Hamiltonians based on two coupled quantum mechanical spins exhibit degenerate eigenvalues despite having no obvious non-abelian symmetries. Operators acting to permute the degenerate states do not have a simple form when expressed as polynomials of the generators of rotations for the respective spins. As observed in [1], one such Hamiltonian helps explain resonances in the spin relaxation rate of optically pumped Rb_2, as a function of applied magnetic field. We give an explanation of why the degeneracies exist, based on properties of the commutator and anti-commutator of the Hamiltonian and its image under magnetic field reversal.

physics.atom-ph↗