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arXiv · 2307.09261

Optical Diffraction Tomography Meets Fluorescence Localization Microscopy

Thanh-An Pham·Emmanuel Soubies·Ferréol Soulez·Michael Unser
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Abstract

We show that structural information can be extracted from single molecule localization microscopy (SMLM) data. More precisely, we reinterpret SMLM data as the measures of a phaseless optical diffraction tomography system for which the illumination sources are fluorophores within the sample. Building upon this model, we propose a joint optimization framework to estimate both the refractive index map and the position of fluorescent molecules from the sole SMLM frames.

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Thanh-An Pham, Emmanuel Soubies, Ferréol Soulez, Michael Unser. 2023-07-18. Optical Diffraction Tomography Meets Fluorescence Localization Microscopy. https://arxiv.org/abs/2307.09261

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