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arXiv · 2405.09601

Fully Automated OCT-based Tissue Screening System

Shaohua Pi·Razieh Ganjee·Lingyun Wang·Riley K. Arbuckle·Chengcheng Zhao·Jose A Sahel·Bingjie Wang·Yuanyuan Chen
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Abstract

This study introduces a groundbreaking optical coherence tomography (OCT) imaging system dedicated for high-throughput screening applications using ex vivo tissue culture. Leveraging OCT's non-invasive, high-resolution capabilities, the system is equipped with a custom-designed motorized platform and tissue detection ability for automated, successive imaging across samples. Transformer-based deep learning segmentation algorithms further ensure robust, consistent, and efficient readouts meeting the standards for screening assays. Validated using retinal explant cultures from a mouse model of retinal degeneration, the system provides robust, rapid, reliable, unbiased, and comprehensive readouts of tissue response to treatments. This fully automated OCT-based system marks a significant advancement in tissue screening, promising to transform drug discovery, as well as other relevant research fields.

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Shaohua Pi, Razieh Ganjee, Lingyun Wang, Riley K. Arbuckle, Chengcheng Zhao, Jose A Sahel, Bingjie Wang, Yuanyuan Chen. 2024-05-15. Fully Automated OCT-based Tissue Screening System. https://doi.org/10.1364/ol.530281

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