Searcharxiv⌕ Search
Home/Archive/arXiv

arXiv · 2406.15621

On the 96-well plate coverglass tilt and curvature suppression in 96-camera imaging system

Antony C Chan
View original source ↗Read source document ↗Export metadata ↓

Abstract

The 96-eyes instrument is capable of computational extended depth of focus (eDOF) of up to +/- 30 micrometer in the phase channel, and conventional depth of field (DOF) of +/- 5 micrometer in the fluorescence channel. However, it requires minimal plate-to-plate cover glass depth variation to function. Plate depths are measured using a third-party plate scanner (Opera Phenix) grouped by plate types (Greiner UV-Star, Cell-Star, and Eppendorf meniscus-free). The two-dimensional (2D) depth dataset is aggregated through principal component analysis to obtain the top eight dominating 2D surface deformation modes. More than 90% of the variation can be explained by the plate's absolute depth and tilt (Pitch, Gradient-Y, and Gradient-X), followed by (~= 2%) the cover glass's curvature (Curve-Y and Curve-XY). Plate-to-plate average depth and tilt variations are suppressed by a customized kinematic mount anchoring the plate's cover glass at the instrument's imaging plane. The plate's average curvature is compensated by manually aligning all 96-eyes microscope objective lenses to track the plate's surface; an one-off calibration procedure aided by the backlash-free piezo-flexure z-stage. Design validation is conducted in silico, with the proof of concept experiment conducted on the 96-eyes with new mounting bracket retrofits.

Explore related subjects

physics.ins-det

Keep this discovery

BibTeXRIS

Antony C Chan. 2024-03-13. On the 96-well plate coverglass tilt and curvature suppression in 96-camera imaging system. https://arxiv.org/abs/2406.15621

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related papers

arXiv2026

High-Speed Semi-FE Readout Module for ATLAS MDT at HL-LHC: Design and Production-Level Characterization

The High-Luminosity upgrade of the Large Hadron Collider (HL-LHC) introduces increased demands on the ATLAS Muon Spectrometer, particularly in terms of data throughput, timing distribution and system reliability. The Phase-II Chamber Service Module (CSM) is a key component of the upgraded Monitored Drift Tube (MDT) trigger and readout system, providing a high-speed interface between the front-end electronics and the backend systems. This paper describes the design and implementation of the Phase-II CSM, together with its validation. The results show that the CSM supports two independent optical uplinks, each operating at a line rate of 10.24 Gbps, together with clock distribution and slow control in the expected operating environment. Integration with small-diameter MDT (sMDT) chambers and tests with the prototype L0MDT trigger system are also presented. The CSM boards are now in production and will be used for installation and integration during the upcoming LHC Long Shutdown.

physics.ins-det↗
arXiv2026

Spectral Discrimination of Deposited Gamma-Ray Energies in a Simulated CeBr$_3$ Scintillator

We show that wavelength measurements of individual detected optical photons may provide additional information about gamma-ray energy deposited in a CeBr$_3$ crystal when the detected-photon-count distributions overlap for nearby gamma-ray energies. Monoenergetic 662 and 629 keV gammas are used in a Geant4 simulation of a $25\times25\times20~\mathrm{mm^3}$ CeBr$_3$ crystal. Assuming a light yield of $6.0\times10^4$ photons/MeV, a wavelength-independent photon-detection efficiency of 30%, and a wavelength resolution of $\sigma_{\lambda}=40$ nm, we find that the fraction of photons reconstructed above 385 nm gives an event-level separation of $\sim$ 2 standard deviations between the 662 and 629 keV event populations selected within the same $\sim$ 1%-wide detected-photon-count interval. No timing or reconstructed interaction-position information is used. The result demonstrates, within the present simulation model, that event-dependent optical spectra can retain energy information beyond an undifferentiated photon count.

physics.ins-det↗
arXiv2026

Operation of a negative ion gas time projection chamber without electronegative fill gases

The high fidelity reconstruction of particle tracks in micropatterned gaseous time projection chambers renders this technology ideal for future rare-event searches, including direction-sensitive dark matter experiments. Large drift distances are typically required for such experiments, so that the overall spatial resolution is limited by diffusion. Negative ion drift exhibits lower diffusion than electron drift and is thus an attractive option for realising a large-scale detector. The use of electronegative gases to create negative ions introduces technical challenges, most notably a reduction in gain when compared to conventional gas mixtures. In this study, we demonstrate a new method for negative ion generation via dissociative electron attachment using the conventional molecular fill gas CF$_4$. Our optical measurements of negative ion drift indicate electron attachment lengths of $<$1 mm and comparable gain to electron avalanches. The individual negative ion avalanches were also time-resolved, allowing the number of ions reaching the readout to be counted. We measure an improved energy resolution by single ion counting, relative to an integrated electron avalanche signal measured under identical gain conditions.

physics.ins-det↗