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arXiv · 2411.07865

Computational refocusing in phase-resolved confocal microscopy

Martin Schnell·Melanie King·Sam Buercklin·Paulo Sarriugarte·Rainer Hillenbrand·P. Scott Carney
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Abstract

We demonstrate numerical refocusing in coherent confocal laser scanning microscopy based on synthetic optical holography. This physics-based approach implements a computational propagation on the complex signal recovered in synthetic holography consistent with the wave physics and the parameters of the microscope. An experimental demonstration is shown to restore an in-focus image of a test object from data acquired at several focal plane off-sets. Numerical refocusing can provide focused views on samples with large height variation with a potential application in confocal optical surface profiling.

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Martin Schnell, Melanie King, Sam Buercklin, Paulo Sarriugarte, Rainer Hillenbrand, P. Scott Carney. 2024-11-12. Computational refocusing in phase-resolved confocal microscopy. https://doi.org/10.1364/ol.496111

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