Investigation of Non-Radiative Relaxation Dynamics Under Pulsed Excitation Using Photon Absorption Remote Sensing: A Proof-of-Principle Study in Mechanical Sensing

Abstract

The mechanical properties of micro-scale bio-entities are fundamental for understanding their functions and pathological states. However, current methods for assessing elastic properties at single-particle level such as Brillouin and atomic force microscopies exhibit intrinsic limitations, including being often slow, having poor resolution, or involving complicated and invasive setups. In this study, we explore Photon Absorption Remote Sensing (PARS) microscopy as a unique solution for mechanical sensing of single micro-objects. PARS uses probe beam scattering/reflectivity measurements to capture non-radiative relaxation process following the absorption of a pulse of light by a micro-object. In particular, we demonstrate that, when operating at GHz-range bandwidth, PARS can trace the sub-nanosecond dynamics of non-radiative relaxation in individual micro-objects, capturing both photoacoustic (PA) pressure propagation and thermal diffusion. This GHz-range measurement, in conjunction with a developed descriptive model, enables the experimental extraction of a minimally distorted PA temporal profile. The PA temporal profile contain information on the ratio between the absorbing object's sound speed and its characteristic diameter, offering a new dimension in PARS microscopy. This enables the assessment of the object's elastic properties, deduced from its speed of sound. Additionally, it offers the potential for sizing objects with known sound speeds. The proof of principle experiments was conducted using spherical polystyrene absorbers, ranging in size from 1 to 10 micrometers with known properties, embedded in a Polydimethylsiloxane (PDMS) matrix. This technique expands the scope of PARS imaging, opening new perspectives for clinical applications in mechanobiology by demonstrating its potential for mechanical imaging.