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arXiv · 2506.20270

Versatile luminescence macroscope with dynamic illumination for photoactive systems

Ian Coghill·Alienor Lahlou·Andrea Lodetti·Shizue Matsubara·Johann Boucle·Thomas Le Saux·Ludovic Jullien
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Abstract

Luminescence imaging is invaluable for studying biological and material systems, particularly when advanced protocols that exploit temporal dynamics are employed. However, implementing such protocols often requires custom instrumentation, either modified commercial systems or fully bespoke setups, which poses a barrier for researchers without expertise in optics, electronics, or software. To address this, we present a versatile macroscopic fluorescence imaging system capable of supporting a wide range of protocols, and provide detailed build instructions along with open-source software to enable replication with minimal prior experience. We demonstrate its broad utility through applications to plants, reversibly photoswitchable fluorescent proteins, and optoelectronic devices.

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Ian Coghill, Alienor Lahlou, Andrea Lodetti, Shizue Matsubara, Johann Boucle, Thomas Le Saux, Ludovic Jullien. 2025-06-25. Versatile luminescence macroscope with dynamic illumination for photoactive systems. https://doi.org/10.1364/oe.570450

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