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arXiv · 2508.13668

Perspective: An outlook on fluorescence tracking

Lance W. Q. Xu·Steve Pressé
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Abstract

Tracking single fluorescent molecules has offered resolution into dynamic molecular processes at the single-molecule level. This perspective traces the evolution of single-molecule tracking, highlighting key developments across various methodological branches within fluorescence microscopy. We compare the strengths and limitations of each approach, ranging from conventional widefield offline tracking to real-time confocal tracking. In the final section, we explore emerging efforts to advance physics-inspired tracking techniques, a possibility for parallelization and artificial intelligence, and discuss challenges and opportunities they present toward achieving higher spatiotemporal resolution and greater computational and data efficiency in next-generation single-molecule studies.

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Lance W. Q. Xu, Steve Pressé. 2025-08-19. Perspective: An outlook on fluorescence tracking. https://doi.org/10.1063/5.0283108

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