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arXiv · 2510.15468

Photothermal Fourier-plane Phase Synchronization for Interferometric Scattering Microscopy

Shupei Lin·Nanfang Jiao·Yevhenii Shaidiuk·Delong Feng·Jingwei Luo·Yihao Yu·Lukasz Bujak·Jianwei Tang·Marek Piliarik·Xue-Wen Chen
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Abstract

We introduce and experimentally implement Fourier-plane phase synchronization for optical microscopy, and demonstrate its performance with interferometric scattering microscopy. By combining a photothermal phase plate and laser beam scanning, we realize a synchronized phase for all scattering components on the Fourier plane of high numerical-aperture microscopes, where the evanescent waves and optical aberration normally produce highly inhomogeneous phase distributions. We achieve an almost perfect point spread function, exhibiting a tighter focus with 50\% enhancement of the signal and ideal circular symmetry. Particularly, by synchronizing the phase to $\pi/2$, we demonstrate the background speckles exhibit an anti-symmetric dependence on axial defocus, enabling the effective suppression of the speckles via defocus integration and thus the detection of 10 nm particles immobilized on the substrate. The concept and technique of seamless dynamic phase control on the Fourier plane constitute a key asset for modern optical microscopy.

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Shupei Lin, Nanfang Jiao, Yevhenii Shaidiuk, Delong Feng, Jingwei Luo, Yihao Yu, Lukasz Bujak, Jianwei Tang, Marek Piliarik, Xue-Wen Chen. 2025-10-17. Photothermal Fourier-plane Phase Synchronization for Interferometric Scattering Microscopy. https://arxiv.org/abs/2510.15468

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