Interfacial Potential Transduction for Diagnostics

Hyun-June Jang; Peuli Nath; Yuqin Wang; Mingoo Kim; Rohit Sai Kodam; Soobin Han; Sangmin Lee; Wookjin Na; Jihoon Kim; Xiaoao Shi; Jeff J. H. Kim; HyunKeun Joo; Byunghoon Ryu; Kiang-Teck Jerry Yeo; Seung-Jung Kee; Howard E. Katz; Junhong Chen; Youngung Seok; Yun Suk Huh; Dino Di Carlo; Hyou-Arm Joung

arXiv · 2603.23775

Interfacial Potential Transduction for Diagnostics

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Abstract

A major barrier to decentralized, near-patient diagnostics is the lack of a signal transduction modality that is both analytically precise and accessible at the point of care. Optical readouts remain instrument-dependent and difficult to miniaturize, while compact electrochemical readouts are prone to matrix-derived signal distortion, limiting their biomarker coverage in real clinical settings. Here, we define interfacial potential transduction as a standardized electrical modality for portable, clinical-grade diagnostics across diverse assay formats. A mechanistic framework identifying key sample matrix parameters within the interfacial potentials transduction system enables control of biofluid-derived interference, and is demonstrated in a widely accessible lateral flow immunoassay format through quantitative detection of estradiol, progesterone, and luteinizing hormone in human plasma with high correlation (r2 > 0.97) to clinical analyzers. Broader applicability across representative diagnostic sectors is further demonstrated through exceptional performance including glucose quantification for biochemical analysis with limit of detection (LOD) of 0.92 ug/dL, HIV p24 capsid protein under an immunomagnetic separation workflow (LOD = 44.8 fg/mL), and hepatitis B virus detection within 5 min via loop-mediated isothermal amplification for molecular diagnostics. Together, these results establish interfacial potentials transduction as a unified diagnostic paradigm for near-patient deployment beyond optical and electrochemical approaches.

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Hyun-June Jang, Peuli Nath, Yuqin Wang, Mingoo Kim, Rohit Sai Kodam, Soobin Han, Sangmin Lee, Wookjin Na, Jihoon Kim, Xiaoao Shi, Jeff J. H. Kim, HyunKeun Joo, Byunghoon Ryu, Kiang-Teck Jerry Yeo, Seung-Jung Kee, Howard E. Katz, Junhong Chen, Youngung Seok, Yun Suk Huh, Dino Di Carlo, Hyou-Arm Joung. 2026-03-24. Interfacial Potential Transduction for Diagnostics. https://arxiv.org/abs/2603.23775

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