arXiv subjects

Andreas Dietzel

Publications and source records attributed to Andreas Dietzel.

3 recordsLinked to original sources

arXiv2026

Smart membrane: high content in situ monitoring barrier on chip with artificial neural network

Conventional transepithelial electrical resistance (TEER) technique provides only a low-content analysis of cell-layer conditions, necessitating repeated microscopic assessments of morphology and cell-cell contacts outside the incubator for barrier-on-chip systems. This work presents a novel high-content TEER device in the form of a novel nanoporous membrane that facilitates continuous electrical measurement of cell-substrate impedance sensing (ECIS). The ultrathin (700 nm) membrane, composed of ultra-low-stress SixNy, is monolithically integrated into wafer-level fabricated chips sealed with glass lids. Coplanar ECIS electrodes were connected to custom electronics to record impedance under sinusoidal excitation. Human umbilical vein endothelial cells (HUVECs) were seeded and continuously recorded impedance spectra were compared with bright-field and fluorescence microscopy, revealing distinct phases of monolayer formation. With one-dimensional convolutional neural network (Conv1d) and Kolmogorov-Arnold Network (KAN) trained with a small amount of Nyquist-diagrams, phases of (I) adherence, (II) outspreading, (III) confluence and (IV) barrier maturity with tight junction formation could be recognized with 95% confidence. As further proof of concept, reversible and irreversible barrier weakening using modulators PN159 and BAC was identified in this way. Our studies have demonstrated that an immediate and automatable non-invasive detection of in-vitro barrier dynamics within barrier-on-chip systems, eliminating the need for microscopy and endpoint staining. We expect this ECIS technique will find broad applications in organ-on-chip systems for in situ monitoring physiological or pathological states of tissue barrier.

eess.SP↗

arXiv2020

Methodology of selective metallic thin film ablation from susceptible polymer substrate using pulsed femtosecond laser

Electronic devices are progressively fabricated on flexible substrates allowing new fields of applications. A maskless and very flexible structuring process is offered by ablation using ultra-short pulse laser irradiation. Usually, certain areas of a functional thin film coating are locally removed to yield the needed device structures. Micro laser patterning quality is not only influenced by the beam properties (beam profile, fluence) but also by pulse overlap, scan repetitions and many other factors such as substrate and coating material. This makes process parameter optimization a challenging task. In this paper, we present a systematic approach to efficiently find suitable parameters for laser-induced ablation of thin films on susceptible polymer substrates. As an example, we use a sputtered NiCr coating with a thickness of 100 $nm$ on a polyimide film made by spin coating of PI-2611 precursor (@ 2000 $rpm$ > ca. 8 $μm$, HD microsystems). Irradiation is conducted using a fs-laser with infrared wavelength of 1030 $nm$ and a pulse length of 212 $fs$. The energy per pulse is varied in the range of 0,29 $μJ$ to 4,83 $μJ$, yielding fluence values between 70 $mJ/cm^2$ and 1,11 $J/cm^2$. Ablation threshold fluence for a 100 $nm$ layer of NiCr was found to be $ϕ_{th}$ ($N_P$:1)=0,50 $J/cm^2$ and the incubation factor was evaluated to be $ξ$=0,53. A clear distinction must be made between the material ablation at the surface of a bulk material and the selective removal of a thin film. In the second case effects such as flaking occur in practice and influence the ablation characteristic. We then investigate different varieties of thin film removal including dot-, line- and areal-ablation. The methodology is presented using a practical example, but can be applied to selective ablation of a wide range of thin film systems.

physics.app-ph↗

arXiv2020

Defining mass transfer in a capillary wave micro-bioreactor

For high-throughput cell culture and associated analytics, droplet-based cultivation systems open up the opportunities for parallelization and rapid data generation. In contrast to microfluidics with continuous flow, sessile droplet approaches enhance the flexibility for fluid manipulation with usually less operational effort. Generating biologically favorable conditions and promoting cell growth in a droplet, however, is particularly challenging due to mass transfer limitations, which has to be solved by implementing an effective mixing technique. Here, capillary waves induced by vertical oscillation are used to mix inside a sessile droplet micro-bioreactor (MBR) system avoiding additional moving parts inside the fluid. Depending on the excitation frequency, different patterns are formed on the oscillating liquid surface, which are described by a model of a vibrated sessile droplet. Analyzing mixing times and oxygen transport into the liquid, a strong dependency of mass transfer on the oscillation parameters, especially the excitation frequency, is demonstrated. Oscillations at distinct capillary wave resonant frequencies lead to rapid homogenization with mixing times of 2 s and volumetric liquid-phase mass transfer coefficients of more than 340 h-1. This shows that the mass transfer in a droplet MBR can be specifically controlled via capillary waves, what is subsequently demonstrated for cultivations of Escherichia coli BL21 cells. Therefore, the presented MBR in combination with vertical oscillation mixing for intensified mass transfer is a promising tool for highly parallel cultivation and data generation.

q-bio.QM↗