arXiv subjects

Chenlei Pang

Publications and source records attributed to Chenlei Pang.

4 recordsLinked to original sources

arXiv2024

3D orientation super-resolution spatial-frequency-shift microscopy

Super-resolution mapping of the 3D orientation of fluorophores reveals the alignment of biological structures where the fluorophores are tightly attached, and thus plays a vital role in studying the organization and dynamics of bio-complexes. However, current super-resolution imaging techniques are either limited to 2D orientation mapping or suffer from slow speed and the requirement of special labels in 3D orientation mapping. Here, we propose a novel polarized virtual spatial-frequency-shift effect to overcome these restrictions to achieve a universal 3D orientation super-resolution mapping capability. To demonstrate the mechanism, we simulate the imaging process and reconstruct the spatial-angular information for sparsely distributed dipoles with random 3D orientations and microfilament-like structures decorated with fluorophores oriented parallel to them. The 3D orientation distribution can be recovered with a doubled spatial resolution and an average angular precision of up to 2.39 degrees. The performance of the approach with noise has also been analyzed considering real implementation.

physics.optics↗

arXiv2022

Single multimode fiber for in vivo light-field encoded nano-imaging

Super-resolution microscopy normally requiring complex and cumbersome optics is not applicable for in situ imaging through a narrow channel. Here, we demonstrate single hair-thin multimode fiber (MMF) endoscope (less than 250 $μm$) for in vivo light-field nano-imaging, which is called spatial-frequency tracking adaptive beacon light-field encoded nano-endoscopy (STABLE nano-endoscopy) that enables three-dimensional (3D) subcellular-scale imaging. Spatial-frequency tracking provides up to $10^3$ Hz disorder tracking that ensures stable imaging in long-haul MMFs (up to 200 m) under various conditions. Full-vector modulation and fluorescence emission difference are combined to enhance the imaging signal-to-noise ratio two times and to improve the resolution to sub-diffraction-limited 250 nm ($λ/3NA$). STABLE nano-endoscopy and white-light endoscopy (WLE) are integrated to achieve cross-scale in vivo imaging inside the lumen. This high-resolution and robust observation in a minimally invasive manner paves the way to gain a deeper understanding of the disease mechanisms and to bridge clinical and biological sciences.

physics.optics↗

arXiv2021

Scalable universal tunable virtual-wavevector spatial frequency shift (TVSFS) super-resolution imaging

Developing a chip-based super-resolution imaging technique with large field-of-view (FOV), deep subwavelength resolution, and compatibility for both fluorescent and non-fluorescent samples is desired for material science, biomedicine, and life researches, etc. Previous on-chip super-resolution methods focus on either fluorescent or non-fluorescent imaging, putting an urgent requirement on the general imaging technique compatible with both of them. Here, we introduce a universal super-resolution imaging method based on tunable virtual-wavevector spatial frequency shift (TVSFS), realizing both labeled and label-free super-resolution imaging on a single delicately fabricated scalable photonic chip. Theoretically, with TVSFS, the diffraction limit of a linear optical system can be overcome, and the resolution can be improved more than three times, which is the limitation for most super-resolution imaging based on spatial frequency engineering. Diffractive units were fabricated on the chip's surface to provide a wavevector-variable evanescent wave illumination and induce tunable deep SFS in the samples' Fourier space. A resolution of λ/4.7 for the label-free sample and λ/7.1 for the labeled sample with a large FOV could be achieved with a CMOS-compatible process on a GaP chip. The large FOV, high-compatibility, and high-integration TVSFS chip may advance the fields like cell engineering, precision inspection in the industry, chemical research, etc.

physics.optics↗

arXiv2019

Wide-field 3D nanoscopy on chip through large and tunable spatial-frequency-shift effect

Linear super-resolution microscopy via synthesis aperture approach permits fast acquisition because of its wide-field implementations, however, it has been limited in resolution because a missing spatial-frequency band occurs when trying to use a shift magnitude surpassing the cutoff frequency of the detection system beyond a factor of two, which causes ghosting to appear. Here, we propose a method of chip-based 3D nanoscopy through large and tunable spatial-frequency-shift effect, capable of covering full extent of the spatial-frequency component within a wide passband. The missing of spatial-frequency can be effectively solved by developing a spatial-frequency-shift actively tuning approach through wave vector manipulation and operation of optical modes propagating along multiple azimuthal directions on a waveguide chip to interfere. In addition, the method includes a chip-based sectioning capability, which is enabled by saturated absorption of fluorophores. By introducing ultra-large propagation effective refractive index, nanoscale resolution is possible, without sacrificing the temporal resolution and the field-of-view. Imaging on GaP waveguide material demonstrates a lateral resolution of lamda/10, which is 5.4 folds above Abbe diffraction limit, and an axial resolution of lamda/19 using 0.9 NA detection objective. Simulation with an assumed propagation effective refractive index of 10 demonstrates a lateral resolution of lamda/22, in which the huge gap between the directly shifted and the zero-order components is completely filled to ensure the deep-subwavelength resolvability. It means that, a fast wide-field 3D deep-subdiffraction visualization could be realized using a standard microscope by adding a mass-producible and cost-effective spatial-frequency-shift illumination chip.

physics.optics↗