R. Heintzmann | Searcharxiv

arXiv2024

Structured illumination microscopy with extreme ultraviolet pulses

The relentless pursuit of understanding matter at ever-finer scales has pushed optical microscopy to surpass the diffraction limit and produced the super-resolution microscopy which enables visualizing structures shorter than the wavelength of light. In the present work, we harnessed extreme ultraviolet beams to create a sub-{\mu}m grating structure, which was revealed by extreme ultraviolet structured illumination microscopy. This achievement marks the first step toward extending such a super-resolution technique into the X-ray regime, where achieving atomic-scale resolution becomes a charming possibility.

physics.optics↗

arXiv2023

Calculating Point Spread Functions: Methods, Pitfalls and Solutions

The knowledge of the exact structure of the optical system PSF enables a high-quality image reconstruction in fluorescence microscopy. Accurate PSF models account for the vector nature of light and the phase and amplitude modifications. Most existing real-space-based PSF models fall into a sampling pitfall near the centre position, yielding to the violation of the energy conservation. In this work, we present novel, to the best of our knowledge, Fourier-based techniques for computing vector PSF and compare them to the state-of-the-art. Our methods are shown to satisfy the physical condition of the imaging process. They are reproducible, computationally efficient, and easy to implement and easy to modify to represent various imaging modalities.

physics.optics↗