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Timo A. Nieminen

Publications and source records attributed to Timo A. Nieminen.

31 records · Page 2Linked to original sources

Collecting single molecules with conventional optical tweezers

The size of particles which can be trapped in optical tweezers ranges from tens of nanometres to tens of micrometres. This size regime also includes large single molecules. Here we present experiments demonstrating that optical tweezers can be used to collect polyethylene oxide (PEO) molecules suspended in water. The molecules that accumulate in the focal volume do not aggregate and therefore represent a region of increased molecule concentration, which can be controlled by the trapping potential. We also present a model which relates the change in concentration to the trapping potential. Since many protein molecules have molecular weights for which this method is applicable the effect may be useful in assisting nucleation of protein crystals.

physics.optics↗

Measurement of refractive index of single microparticles

The refractive index of single microparticles is derived from precise measurement and rigorous modeling of the stiffness of a laser trap. We demonstrate the method for particles of four different materials with diameters from 1.6 to 5.2 microns and achieve an accuracy of better than 1%. The method greatly contributes as a new characterization technique because it works best under conditions (small particle size, polydispersion) where other methods, such as absorption spectroscopy, start to fail. Particles need not be transferred to a particular fluid, which prevents particle degradation or alteration common in index matching techniques. Our results also show that advanced modeling of laser traps accurately reproduces experimental reality.

physics.optics↗

Modelling optical micro-machines

A strongly focused laser beam can be used to trap, manipulate and exert torque on a microparticle. The torque is the result of transfer of angular momentum by scattering of the laser beam. The laser could be used to drive a rotor, impeller, cog wheel or some other microdevice of a few microns in size, perhaps fabricated from a birefringent material. We review our methods of computationally simulating the torque and force imparted by a laser beam. We introduce a method of hybridizing the T-matrix with the Finite Difference Frequency Domain (FDFD) method to allow the modelling of materials that are anisotropic and inhomogeneous, and structures that have complex shapes. The high degree of symmetry of a microrotor, such as discrete or continuous rotational symmetry, can be exploited to reduce computational time and memory requirements by orders of magnitude. This is achieved by performing calculations for only a given segment or plane that is repeated across the whole structure. This can be demonstrated by modelling the optical trapping and rotation of a cube.

physics.optics↗

Measurement of the total optical angular momentum transfer in optical tweezers

We describe a way to determine the total angular momentum, both spin and orbital, transferred to a particle trapped in optical tweezers. As an example an LG02 mode of a laser beam with varying degrees of circular polarisation is used to trap and rotate an elongated particle with a well defined geometry. The method successfully estimates the total optical torque applied to the particle. For this technique, there is no need to measure the viscous drag on the particle, as it is an optical measurement. Therefore, knowledge of the particle's size and shape, as well as the fluid's viscosity, is not required.

physics.optics↗

Orientation of optically trapped nonspherical birefringent particles

While the alignment and rotation of microparticles in optical traps have received increased attention recently, one of the earliest examples has been almost totally neglected the alignment of particles relative to the beam axis, as opposed to about the beam axis. However, since the alignment torques determine how particles align in a trap, they are directly relevant to practical applications. Lysozyme crystals are an ideal model system to study factors determining the orientation of nonspherical birefringent particles in a trap. Both their size and their aspect ratio can be controlled by the growth parameters, and their regular shape makes computational modeling feasible. We show that both external shape and internal birefringence anisotropy contribute to the alignment torque. Three-dimensionally trapped elongated objects either align with their long axis parallel or perpendicular to the beam axis depending on their size. The shape-dependent torque can exceed the torque due to birefringence, and can align negative uniaxial particles with their optic axis parallel to the electric field, allowing an application of optical torque about the beam axis.

physics.optics↗

Non-steady-state double-resonant extremely asymmetrical scattering of waves in periodic gratings

Double-resonant extremely asymmetrical scattering (DEAS) is a strongly resonant type of Bragg scattering in two joint or separated uniform gratings with different phases. It is characterised by a very strong increase of the scattered and incident wave amplitudes inside and between the gratings at a resonant phase shift between the gratings. DEAS is realised when the first diffracted order satisfying the Bragg condition propagates parallel to the grating boundaries, and the joint or separated gratings interact by means of the diffractional divergence of the scattered waves from one grating into another. This Letter develops a theory of non-steady-state DEAS of bulk TE electromagnetic waves in holographic gratings, and investigates the process of relaxation of the incident and scattered wave amplitudes to their steady-state values inside and outside the gratings. Typical relaxation times are determined. Physical explanation of the predicted effects is presented.

physics.optics↗

Anomalous absorption of bulk shear sagittal acoustic waves in a layered structure with viscous fluid

It is demonstrated theoretically that the absorptivity of bulk shear sagittal waves by an ultra-thin layer of viscous fluid between two different elastic media has a strong maximum (in some cases as good as 100%) at an optimal layer thickness. This thickness is usually much smaller than the penetration depths and lengths of transverse and longitudinal waves in the fluid. The angular dependencies of the absorptivity are demonstrated to have significant and unusual structure near critical angles of incidence. The effect of non-Newtonian properties and non-uniformities of the fluid layer on the absorptivity is also investigated. In particular, it is shown that the absorption in a thin layer of viscous fluid is much more sensitive to non-zero relaxation time(s) in the fluid layer than the absorption at an isolated solid-fluid interface.

physics.class-ph↗

Optical measurement of torque exerted on an elongated object by a non-circular laser beam

We have developed a scheme to measure the optical torque, exerted by a laser beam on a phase object, by measuring the orbital angular momentum of the transmitted beam. The experiment is a macroscopic simulation of a situation in optical tweezers, as orbital angular momentum has been widely used to apply torque to microscopic objects. A hologram designed to generate LG02 modes and a CCD camera are used to detect the orbital component of the beam. Experimental results agree with theoretical numerical calculations, and the strength of the orbital component suggest its usefulness in optical tweezers for micromanipulation.

physics.optics↗

Angular momentum of a strongly focussed Gaussian beam

A circularly polarized rotationally symmetric paraxial laser beams carries hbar angular momentum per photon as spin. Focussing the beam with a rotationally symmetric lens cannot change this angular momentum flux, yet the focussed beam must have spin less than hbar per photon. The remainder of the original spin is converted to orbital angular momentum, manifesting itself as a longitudinal optical vortex at the focus. This demonstrates that optical orbital angular momentum can be generated by a rotationally symmetric optical system which preserves the total angular momentum of the beam.

physics.optics↗

Optical microrheology using rotating laser-trapped particles

We demonstrate an optical system that can apply and accurately measure the torque exerted by the trapping beam on a rotating birefringent probe particle. This allows the viscosity and surface effects within liquid media to be measured quantitatively on a micron-size scale using a trapped rotating spherical probe particle. We use the system to measure the viscosity inside a prototype cellular structure.

physics.optics↗

Comment on "Geometric absorption of electromagnetic angular momentum", C. Konz, G. Benford

The core theorem on which the above paper is centred - that a perfectly conducting body of revolution absorbs no angular momentum from an axisymmetric electromagnetic wave field - is in fact a special case of a more general result in electromagnetic scattering theory. In addition, the scaling of the efficiency of transfer of angular momentum to an object with the wavelength and object size merits further discussion. Finally, some comments are made on the choice of terminology and the erroneous statement that a circularly polarized plane wave does not carry angular momentum.

physics.optics↗

Non-steady-state extremely asymmetrical scattering of waves in periodic gratings

Extremely asymmetrical scattering (EAS) is a highly resonant type of Bragg scattering with a strong resonant increase of the scattered wave amplitude inside and outside the grating. EAS is realized when the scattered wave propagates parallel to the grating boundaries. We present a rigorous algorithm for the analysis of non-steady-state EAS, and investigate the relaxation of the incident and scattered wave amplitudes to their steady-state values. Non-steady-state EAS of bulk TE electromagnetic waves is analyzed in narrow and wide, slanted, holographic gratings. Typical relaxation times are determined and compared with previous rough estimations. Physical explanation of the predicted effects is presented.

physics.optics↗

Optical application and measurement of torque on microparticles of isotropic nonabsorbing material

We show how it is possible to controllably rotate or align microscopic particles of isotropic nonabsorbing material in a TEM00 Gaussian beam trap, with simultaneous measurement of the applied torque using purely optical means. This is a simple and general method of rotation, requiring only that the particle is elongated along one direction. Thus, this method can be used to rotate or align a wide range of naturally occurring particles. The ability to measure the applied torque enables the use of this method as a quantitative tool--the rotational equivalent of optical tweezers based force measurement. As well as being of particular value for the rotation of biological specimens, this method is also suitable for the development of optically-driven micromachines.

physics.optics↗