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arXiv · 0911.5040

Imaging Gold Nanoparticles in Living Cells Environments using Heterodyne Digital Holographic Microscopy

Nilanthi Warnasooriya·Fadwa Joud·Philippe Bun·Gilles Tessier·Maite Coppey-Moisan·Pierre Desbiolles·Michael Atlan·Marie Abboud·Michel Gross
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Abstract

This paper describes an imaging microscopic technique based on heterodyne digital holography where subwavelength-sized gold colloids can be imaged in cell environment. Surface cellular receptors of 3T3 mouse fibroblasts are labeled with 40 nm gold nanoparticles, and the biological specimen is imaged in a total internal reflection configuration with holographic microscopy. Due to a higher scattering efficiency of the gold nanoparticles versus that of cellular structures, accurate localization of a gold marker is obtained within a 3D mapping of the entire sample's scattered field, with a lateral precision of 5 nm and 100 nm in the x,y and in the z directions respectively, demonstrating the ability of holographic microscopy to locate nanoparticles in living cells environments.

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Nilanthi Warnasooriya, Fadwa Joud, Philippe Bun, Gilles Tessier, Maite Coppey-Moisan, Pierre Desbiolles, Michael Atlan, Marie Abboud, Michel Gross. 2009-11-26. Imaging Gold Nanoparticles in Living Cells Environments using Heterodyne Digital Holographic Microscopy. https://doi.org/10.1364/oe.18.003264

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