Femtosecond Photonic Viral Inactivation Probed Using Solid-State Nanopores

Mina Nazari; Xiaoqing Li; Mohammad Amin Alibakhshi; Haojie Yang; Kathleen Souza; Christopher Gillespie; Suryaram Gummuluru; Björn M. Reinhard; Kirill S. Korolev; Lawrence D. Ziegler; Qing Zhao; Meni Wanunu; Shyamsunder Erramilli

arXiv · 1806.01463

Femtosecond Photonic Viral Inactivation Probed Using Solid-State Nanopores

Mina Nazari·Xiaoqing Li·Mohammad Amin Alibakhshi·Haojie Yang·Kathleen Souza·Christopher Gillespie·Suryaram Gummuluru·Björn M. Reinhard·Kirill S. Korolev·Lawrence D. Ziegler·Qing Zhao·Meni Wanunu·Shyamsunder Erramilli

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Abstract

We report on the detection of inactivation of virus particles using femtosecond laser radiation by measuring the conductance of a solid state nanopore designed for detecting single virus particles. Conventional methods of assaying for viral inactivation based on plaque forming assays require 24-48 hours for bacterial growth. Nanopore conductance measurements provide information on morphological changes at a single virion level. We show that analysis of a time series of nanopore conductance can quantify the detection of inactivation, requiring only a few minutes from collection to analysis. Morphological changes were verified by Dynamic Light Scattering (DLS). Statistical analysis maximizing the information entropy provides a measure of the Log-reduction value. Taken together, our work provides a rapid method for assaying viral inactivation with femtosecond lasers using solid-state nanopores.

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Mina Nazari, Xiaoqing Li, Mohammad Amin Alibakhshi, Haojie Yang, Kathleen Souza, Christopher Gillespie, Suryaram Gummuluru, Björn M. Reinhard, Kirill S. Korolev, Lawrence D. Ziegler, Qing Zhao, Meni Wanunu, Shyamsunder Erramilli. 2018-06-05. Femtosecond Photonic Viral Inactivation Probed Using Solid-State Nanopores. https://arxiv.org/abs/1806.01463

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