Force and kinetics of fast and slow muscle myosin determined with a synthetic sarcomere-like nanomachine

Valentina Buonfiglio; Irene Pertici; Matteo Marcello; Ilaria Morotti; Marco Caremani; Massimo Reconditi; Marco Linari; Duccio Fanelli; Vincenzo Lombardi; Pasquale Bianco

arXiv · 2408.00373

Force and kinetics of fast and slow muscle myosin determined with a synthetic sarcomere-like nanomachine

Valentina Buonfiglio·Irene Pertici·Matteo Marcello·Ilaria Morotti·Marco Caremani·Massimo Reconditi·Marco Linari·Duccio Fanelli·Vincenzo Lombardi·Pasquale Bianco

View original source ↗Read source document ↗Export metadata ↓

Abstract

Myosin II is the muscle molecular motor that works in two bipolar arrays in each thick filament of the striated (skeletal and cardiac) muscle, converting the chemical energy into steady force and shortening by cyclic ATP--driven interactions with the nearby actin filaments. Different isoforms of the myosin motor in the skeletal muscles account for the different functional requirements of the slow muscles (primarily responsible for the posture) and fast muscles (responsible for voluntary movements). To clarify the molecular basis of the differences, here the isoform--dependent mechanokinetic parameters underpinning the force of slow and fast muscles are defined with a unidimensional synthetic nanomachine powered by pure myosin isoforms from either slow or fast rabbit skeletal muscle. Data fitting with a stochastic model provides a self--consistent estimate of all the mechanokinetic properties of the motor ensemble including the motor force, the fraction of actin--attached motors and the rate of transition through the attachment--detachment cycle. The achievements in this paper set the stage for any future study on the emergent mechanokinetic properties of an ensemble of myosin molecules either engineered or purified from mutant animal models or human biopsies.

Explore related subjects

cond-mat.stat-mech q-bio.BM q-bio.PE q-bio.TO

Keep this discovery

Explore connections, maps & timelines

BibTeX RIS

Valentina Buonfiglio, Irene Pertici, Matteo Marcello, Ilaria Morotti, Marco Caremani, Massimo Reconditi, Marco Linari, Duccio Fanelli, Vincenzo Lombardi, Pasquale Bianco. 2024-08-01. Force and kinetics of fast and slow muscle myosin determined with a synthetic sarcomere-like nanomachine. https://arxiv.org/abs/2408.00373

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related papers

arXiv2026

Universal sampling of spin systems across quenched disorder

Statistical physics extracts macroscopic laws by averaging over the many microscopic degrees of freedom of a system. Disordered systems demand a second and far harder average, one over the quenched randomness itself. The classic analytical routes, the replica and cavity methods, become uncontrolled outside mean-field or tree-like limits, and conventional numerical algorithms like parallel tempering require expensive, independent equilibration for every disorder realization. In this work, we introduce a universal neural variational framework that amortizes inference across the disorder ensemble, eliminating both the need for per-instance Markov chain equilibration and the cost of retraining instance-specific variational ansatzes. Built on an encoder-decoder Transformer architecture, after training once, it produces an explicit approximation to the Boltzmann distribution given previously unseen disorder realizations without further optimization. We validate this framework on 2D Edwards-Anderson models, and apply it to the random-bond Ising model, successfully capturing the Binder cumulant crossings near the Nishimori multicritical point. These results shift the object of variational inference from the single instance to the disorder ensemble, opening a route to frustrated many-body systems where instance-by-instance computation is prohibitive.

cond-mat.stat-mech↗

arXiv2026

Information-Theoretic Characterization of Macroscopic Chaos Emerging from the Chemical Master Equation

Open chemical reaction networks exhibit stochastic concentration dynamics at finite system sizes, whereas their macroscopic limit is governed by deterministic rate equations that can display chaos. In this Letter, we show theoretically that a rate of information loss constructed from two-time mutual information recovers the Kolmogorov-Sinai entropy in the deterministic limit. We verify this result through numerical simulations of a Markov jump process for a three-species system involving seven reactions.

cond-mat.stat-mech↗

arXiv2026

Orientational order on non-orientable domains

We study the statistical properties of passive and active many-body systems with orientational degrees of freedom on non-orientable domains. By rephrasing topological constraints as non-local symmetry relations on an orientable double-cover, we show that non-orientability eliminates global rotational soft modes without acting like an external field. In a passive XY model, this results in topological caging, where orientational fluctuations that exhibit conventional diffusive behavior on a torus saturate on a Klein bottle to a finite value that we compute exactly in the thermodynamic limit. In models of active self-propelled particles with orientational degrees of freedom, topological caging persists despite continuously changing interaction neighborhoods. In an active Ising spin model, non-orientability enforces the coexistence of ordered anti-parallel domains with vanishing global polar order, a state that is absent on orientable domains.

cond-mat.stat-mech↗