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Jerome Mertz

Publications and source records attributed to Jerome Mertz.

23 records · Page 2Linked to original sources

High-throughput Imaging of Self-luminous Objects through a Single Optical Fiber

Imaging through a single optical fiber offers attractive possibilities in many applications such as microendoscopy or remote sensing. However, the direct transmission of an image through an optical fiber is difficult because spatial information is scrambled upon propagation. We demonstrate an image transmission strategy where spatial information is first converted to spectral information. Our strategy is based on a principle of spread-spectrum encoding, borrowed from wireless communications, wherein object pixels are converted into distinct spectral codes that span the full bandwidth of the object spectrum. Image recovery is performed by numerical inversion of the detected spectrum at the fiber output. We provide a simple demonstration of spread-spectrum encoding using Fabry-Perot etalons. Our technique enables the 2D imaging of self-luminous (i.e. incoherent) objects with high throughput in principle independent of pixel number. Moreover, it is insensitive to fiber bending, contains no moving parts, and opens the possibility of extreme miniaturization.

physics.optics↗

Field of view advantage of conjugate adaptive optics in microscopy applications

The imaging performance of an optical microscope can be degraded by sample-induced aberrations. A general strategy to undo the effect of these aberrations is to apply wavefront correction with a deformable mirror (DM). In most cases, the DM is placed conjugate to the microscope pupil, called pupil adaptive optics (AO). When the aberrations are spatially variant, an alternative configuration involves placing the DM conjugate to the main source of aberrations, called conjugate AO. We provide theoretical and experimental comparison of both configurations for the simplified case where spatially variant aberrations are produced by a well defined phase screen. We pay particular attention to the resulting correction field of view (FOV). Conjugate AO is found to provide a significant FOV advantage. While this result is well known in the astronomy community, our goal here is to recast it specifically for the optical microscopy community.

physics.optics↗

Single-exposure profilometry using partitioned aperture wavefront imaging

We demonstrate a technique for instantaneous measurements of surface topography based on the combination of a partitioned aperture wavefront imager with a standard lamp-based reflection microscope. The technique can operate at video rate over large fields of view, and provides nanometer axial resolution and sub-micron lateral resolution. We discuss performance characteristics of this technique, which we experimentally compare with scanning white light interferometry.

physics.optics↗

Focusing polychromatic light through strongly scattering media

We demonstrate feedback-optimized focusing of spatially coherent polychromatic light after transmission through strongly scattering media, and describe the relationship between optimized focus intensity and initial far-field speckle contrast. Optimization is performed using a MEMS spatial light modulator with camera-based or spectrometer-based feedback. We observe that the spectral bandwidth of the optimized focus depends on characteristics of the feedback signal. We interpret this dependence as a modification in the number of spectral modes transmitted by the sample, and introduce a simple model for polychromatic focus enhancement that is corroborated by experiment with calibrated samples.

physics.optics↗

Phase gradient microscopy in thick tissue with oblique back-illumination

Phase contrast techniques, such as differential interference contrast (DIC) microscopy, are widely used to provide morphological images of unstained biological samples. The trans-illumination geometry required for these techniques has restricted their application to thin samples. We introduce oblique back-illumination microscopy (OBM), a method of collecting en face phase gradient images of thick scattering samples, enabling near video-rate in vivo phase imaging with a miniaturized probe suitable for endoscopy.

physics.optics↗