arXiv subjects
Publications and source records attributed to Michael Atlan.
We report a demonstration video-rate heterodyne holography in off-axis configuration. Reconstruction and display of 1 Megapixel holograms is achieved at 24 frames per second, with a graphics processing unit. Our claims are validated with real-time screening of steady-state vibration amplitudes in a wide-field, non-contact vibrometry experiment.
This Letter reports a demonstration of off-axis compressed holography in low-light level imaging conditions. An acquisition protocol relying on a single exposure of a randomly undersampled diffraction map of the optical field, recorded in the high heterodyne gain regime, is proposed. The image acquisition scheme is based on compressed sensing, a theory establishing that near-exact recovery of an unknown sparse signal is possible from a small number of nonstructured measurements. Image reconstruction is further enhanced by introducing an off-axis spatial support constraint to the image estimation algorithm. We report accurate experimental recovering of holographic images of a resolution target in low-light conditions with a frame exposure of 5 μs, scaling down measurements to 9% of random pixels within the array detector.
We report laser Doppler ophthalmoscopic fundus imaging in the rat eye with near-IR heterodyne holography. Sequential sampling of the beat of the reflected radiation against a frequency-shifted optical local oscillator is made onto an array detector. Wide-field maps of fluctuation spectra in the 10 Hz to 25 kHz band exhibit angiographic contrasts in the retinal vascular tree without requirement of an exogenous marker.
This work reveals an experimental microscopy acquisition scheme successfully combining Compressed Sensing (CS) and digital holography in off-axis and frequency-shifting conditions. CS is a recent data acquisition theory involving signal reconstruction from randomly undersampled measurements, exploiting the fact that most images present some compact structure and redundancy. We propose a genuine CS-based imaging scheme for sparse gradient images, acquiring a diffraction map of the optical field with holographic microscopy and recovering the signal from as little as 7% of random measurements. We report experimental results demonstrating how CS can lead to an elegant and effective way to reconstruct images, opening the door for new microscopy applications.
We developed a microscope intended to probe, using a parallel heterodyne receiver, the fluctuation spectrum of light quasi-elastically scattered by gold nanoparticles diffusing in viscous fluids. The cutoff frequencies of the recorded spectra scale up linearly with those expected from single scattering formalism in a wide range of dynamic viscosities (1 to 15 times water viscosity at room temperature). Our scheme enables ensemble-averaged optical fluctuations measurements over multispeckle recordings in low light, at temporal frequencies up to 10 kHz, with a 12 Hz framerate array detector.
This paper describes an imaging microscopic technique based on heterodyne digital holography where subwavelength-sized gold colloids can be imaged in cell environment. Surface cellular receptors of 3T3 mouse fibroblasts are labeled with 40 nm gold nanoparticles, and the biological specimen is imaged in a total internal reflection configuration with holographic microscopy. Due to a higher scattering efficiency of the gold nanoparticles versus that of cellular structures, accurate localization of a gold marker is obtained within a 3D mapping of the entire sample's scattered field, with a lateral precision of 5 nm and 100 nm in the x,y and in the z directions respectively, demonstrating the ability of holographic microscopy to locate nanoparticles in living cells environments.
We describe a scheme into which a camera is turned into an efficient tunable frequency filter of a few Hertz bandwidth in an off-axis, heterodyne optical mixing configuration, enabling to perform parallel, high-resolution coherent spectral imaging. This approach is made possible through the combination of a spatial and temporal modulation of the signal to reject noise contributions. Experimental data obtained with dynamically scattered light by a suspension of particles in brownian motion is interpreted.
We obtain quantitative measurements of the oscillation amplitude of vibrating objects by using sideband digital holography. The frequency sidebands on the light scattered by the object, shifted by n times the vibration frequency, are selectively detected by heterodyne holography, and images of the object are calculated for different orders n. Orders up to n=120 have been observed, allowing the measurement of amplitudes of oscillation that are significantly larger than the optical wavelength. Using the positions of the zeros of intensity for each value of n, we reconstruct the shape of vibration the object.
We report the first observation of the dependence of the coherent-backscattering (CBS) enhanced cone with the frequency of the backscattered photon. The experiment is performed on a diffusing liquid suspension and the Doppler broadening of light is induced by the Brownian motion of the scatterers. Heterodyne detection on a CCD camera is used to measure the complex field (i.e., the hologram) of the light that is backscattered at a given frequency. The analysis of the holograms yield the frequency and the propagation direction of the backscattered photons. We observe that the angular CBS cone becomes more narrow in the tail of the Brownian spectrum. The experimental results are in good agreement with a simple theoretical model.
We have compared the respective efficiencies of off-axis and phase-shifting holography in terms of noise and aliases removal. The comparison is made by analyzing holograms of an USAF target backlit with laser illumination, recorded with a charge-coupled device camera. We show that it is essential to remove the LO beam noise, especially at low illumination levels.
We propose a variant of the heterodyne holography scheme that combines the properties of off-axis and phase-shifting holography. This scheme makes it possible to filter off numerically the zero-order image alias and the technical noise of the reference. It is then possible to record and reconstruct holographic images at an extremely low signal level. We show experimentally that the sensitivity of the method is limited only by the quantum nature of photons.
We report experimental results of parallel measurement of spectral components of the light. The temporal fluctuations of an optical field mixed with a separate reference are recorded with a high throughput complementary metal oxide semi-conductor camera (1 Megapixel at 2 kHz framerate). A numerical Fourier transform of the time-domain recording enables wide-field coherent spectral imaging. Qualitative comparisons with frequency-domain wide-field laser Doppler imaging are provided.
We report experimental results on heterodyne holographic microscopy of subwavelength-sized gold particles. The apparatus uses continuous green laser illumination of the metal beads in a total internal reflection configuration for dark-field operation. Detection of the scattered light at the illumination wavelength on a charge-coupled device array detector enables 3D localization of brownian particles in water