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arXiv · 2203.13630

Bond-Selective Intensity Diffraction Tomography

Jian Zhao·Alex Matlock·Hongbo Zhu·Ziqi Song·Jiabei Zhu·Biao Wang·Fukai Chen·Yuewei Zhan·Zhicong Chen·Yihong Xu·Xingchen Lin·Lei Tian·Ji-Xin Cheng
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Abstract

Recovering molecular information remains a grand challenge in the widely used holographic and computational imaging technologies. To address this challenge, we developed a computational mid-infrared photothermal microscope, termed Bond-selective Intensity Diffraction Tomography (BS-IDT). Based on a low-cost brightfield microscope with an add-on pulsed light source, BS-IDT recovers both infrared spectra and bond-selective 3D refractive index maps from intensity-only measurements. High-fidelity infrared fingerprint spectra extraction is validated. Volumetric chemical imaging of biological cells is demonstrated at a speed of ~20 seconds per volume, with a lateral and axial resolution of ~350 nm and ~1.1 micron, respectively. BS-IDT's application potential is investigated by chemically quantifying lipids stored in cancer cells and volumetric chemical imaging on Caenorhabditis elegans with a large field of view (~100 micron X 100 micron).

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Jian Zhao, Alex Matlock, Hongbo Zhu, Ziqi Song, Jiabei Zhu, Biao Wang, Fukai Chen, Yuewei Zhan, Zhicong Chen, Yihong Xu, Xingchen Lin, Lei Tian, Ji-Xin Cheng. 2022-03-25. Bond-Selective Intensity Diffraction Tomography. https://doi.org/10.1038/s41467-022-35329-8

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