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arXiv · 2604.21186

Nanoscale Fluorescence Thermometry: Probes, Recent Advances and Emerging Directions

Md Shakhawath Hossain·Nhat Minh Nguyen·Thi Ngoc Anh Mai·Trung Vuong Doan·Chaohao Chen·Qian Peter Su·Jiayan Liao·Yongliang Chen·Quynh Le-Van·Vu Khac Dat·Toan Dinh·Xiaoxue Xu·Toan Trong Tran
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Abstract

The transition of materials and devices to nanometer, atomic, and quantum scales makes thermal characterization increasingly challenging, driving the need for advanced nanoscale thermometry. Fluorescence nanothermometry has emerged as a powerful approach, enabling remote, spatially resolved temperature measurements with sub-micrometer-to-nanometer precision across applications in nanoelectronics, microfluidics, and biological systems. In these systems, temperature is inferred from variations in fluorescence observables, including spectral position, intensity, linewidth, and excited-state dynamics. This review provides a comprehensive and critical overview of fluorescence nanothermometry, covering fundamental mechanisms, material platforms, recent advances, and emerging applications. It further presents a critical evaluation of key challenges and discusses emerging strategies and future research directions toward achieving robust, real-time thermometry. It is anticipated that this review will stimulate further advances in material platforms and system design, accelerating the development of accurate, scalable, and application-ready nanoscale thermometers.

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Md Shakhawath Hossain, Nhat Minh Nguyen, Thi Ngoc Anh Mai, Trung Vuong Doan, Chaohao Chen, Qian Peter Su, Jiayan Liao, Yongliang Chen, Quynh Le-Van, Vu Khac Dat, Toan Dinh, Xiaoxue Xu, Toan Trong Tran. 2026-04-23. Nanoscale Fluorescence Thermometry: Probes, Recent Advances and Emerging Directions. https://arxiv.org/abs/2604.21186

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